| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,809,581 | G→A | 55.8% | G52G (GGG→GGA) | mprA → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,809,581 | 0 | G | A | 55.8% | 23.9 / 120.9 | 86 | G52G (GGG→GGA) | mprA | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (20/18); new base A (24/24); total (44/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
CTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTT > W3110S.gb/2809514‑2809648 | cTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGAtt < 1:981777/71‑1 (MQ=255) tCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACg < 1:2263159/71‑1 (MQ=255) tttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATAAAc > 1:555719/1‑68 (MQ=255) tttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAAc > 1:1144491/1‑68 (MQ=255) ttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACgaga > 1:553149/1‑71 (MQ=255) gcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACg > 1:1108752/1‑71 (MQ=255) gcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACg > 1:628918/1‑71 (MQ=255) gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt > 1:1871685/1‑71 (MQ=255) gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt > 1:1597927/1‑71 (MQ=255) gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt > 1:2221692/1‑71 (MQ=255) cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt < 1:172931/71‑1 (MQ=255) acatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt > 1:1323928/1‑70 (MQ=255) acatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGttt < 1:1218010/71‑1 (MQ=255) gcaAAGCAAGCTGCTGTAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTa > 1:906099/1‑68 (MQ=255) gcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgaga > 1:730540/1‑59 (MQ=255) gcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttg > 1:2146348/1‑64 (MQ=255) gcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATgg > 1:2399935/1‑71 (MQ=255) gcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:1651167/1‑68 (MQ=255) gcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATg > 1:397538/1‑70 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt > 1:2424891/1‑68 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGc > 1:1510491/1‑71 (MQ=255) aaaGCAAGCTTCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt > 1:505145/1‑67 (MQ=255) gcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTa > 1:2569813/1‑48 (MQ=255) gcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgt > 1:2477165/1‑57 (MQ=255) gcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGtt < 1:1610675/70‑1 (MQ=255) caagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:1412858/46‑1 (MQ=255) caagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACg > 1:1923088/1‑50 (MQ=255) agcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACTAGACgttgtt < 1:465684/58‑1 (MQ=255) gctgctGGAGAACCGCAATAAAATGCTGAAGGCTCATGGAAtt < 1:4217/43‑1 (MQ=38) tgctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACg > 1:2314301/1‑71 (MQ=255) ctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc > 1:636159/1‑42 (MQ=255) ctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACg < 1:798937/69‑1 (MQ=255) tgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTAc < 1:2024243/67‑1 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg < 1:2254296/69‑1 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg < 1:1356967/69‑1 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGa < 1:1533531/71‑1 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGa < 1:267926/71‑1 (MQ=255) gaACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGt > 1:140166/1‑71 (MQ=255) gaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGAc > 1:225469/1‑42 (MQ=255) aaCCGCAATAAAATGCTGAAGGCTCAGGGAATTaa < 1:1077402/35‑1 (MQ=255) aaCCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGAt < 1:2339465/59‑1 (MQ=255) aaCCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtc > 1:1251717/1‑71 (MQ=255) aCCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct < 1:1376265/71‑1 (MQ=255) gCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt < 1:219134/55‑1 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGttt > 1:1020368/1‑43 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGa > 1:1011927/1‑63 (MQ=255) aaTAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGa < 1:2260652/71‑1 (MQ=255) aTAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGa > 1:2590057/1‑51 (MQ=255) aTAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGa > 1:2342284/1‑70 (MQ=255) aTAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGa > 1:1618071/1‑70 (MQ=255) tAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt < 1:262015/48‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaa < 1:983611/71‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaa < 1:1761228/71‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACgt < 1:1007046/35‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGAt < 1:843864/51‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTa < 1:737841/53‑1 (MQ=255) aaaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt > 1:856892/1‑50 (MQ=255) aaaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGa > 1:857661/1‑49 (MQ=255) aaaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTTCGCTGGAGTCTCAGGaaaa > 1:2253412/1‑71 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGa > 1:261605/1‑48 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGa > 1:2624132/1‑48 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACg > 1:1900778/1‑53 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa > 1:814545/1‑68 (MQ=255) aaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt < 1:1227064/48‑1 (MQ=255) tGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt < 1:2037732/35‑1 (MQ=255) cTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACcaca < 1:931131/70‑1 (MQ=255) cTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAg > 1:182890/1‑71 (MQ=255) cTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAg > 1:734609/1‑71 (MQ=255) cTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAg < 1:1205582/71‑1 (MQ=255) cTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAg > 1:1281599/1‑71 (MQ=255) gAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGAt < 1:435483/42‑1 (MQ=255) gAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGAt < 1:334928/42‑1 (MQ=255) aaGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGa > 1:1595071/1‑40 (MQ=255) aaGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAt < 1:1902688/71‑1 (MQ=255) aaGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGa > 1:666124/1‑40 (MQ=255) aaGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg < 1:118035/58‑1 (MQ=255) aGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAtt < 1:2258024/71‑1 (MQ=255) gCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACg > 1:1106965/1‑42 (MQ=255) cTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACcaca < 1:620867/63‑1 (MQ=255) cTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAg < 1:516416/71‑1 (MQ=255) cTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAg < 1:1398415/71‑1 (MQ=255) tCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa < 1:1085161/55‑1 (MQ=255) tCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa < 1:1063022/55‑1 (MQ=255) tCAGGGAATTAACGAGACGTTGTTTATGGCGTTGa < 1:1674386/35‑1 (MQ=255) tCAGGGAATTAACGAGACGTTGTTTATGGCGTTGa < 1:2638093/35‑1 (MQ=255) tCAGGGAATTAACGAGACGTTGTTTATGGCGTTGa < 1:1088907/35‑1 (MQ=255) gggtATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaa < 1:1905318/53‑1 (MQ=255) ggggATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg < 1:36579/40‑1 (MQ=255) gggaATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCtt < 1:2597364/71‑1 (MQ=255) gggATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa < 1:736908/51‑1 (MQ=255) | CTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTT > W3110S.gb/2809514‑2809648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |