| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,053,471 | A→G | 21.9% | F9S (TTT→TCT) | pepP ← | proline aminopeptidase P II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,053,471 | 0 | A | G | 21.9% | 61.0 / 15.4 | 32 | F9S (TTT→TCT) | pepP | proline aminopeptidase P II |
| Reads supporting (aligned to +/- strand): ref base A (14/11); new base G (3/4); total (17/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01 | |||||||||||
CAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTA > W3110S.gb/3053406‑3053529 | cagcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAGActct > 1:1426299/1‑71 (MQ=255) aTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGa < 1:1637929/71‑1 (MQ=255) aTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGa < 1:2041545/71‑1 (MQ=255) cggcgCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCgg < 1:1399241/62‑1 (MQ=255) ggcgCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATctcac > 1:846450/1‑71 (MQ=255) cgcTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATctcact > 1:715716/1‑70 (MQ=255) cGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAac < 1:238713/70‑1 (MQ=255) ttGCATTTGCTTCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCgg < 1:927070/49‑1 (MQ=255) gCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACtctc > 1:2047541/1‑70 (MQ=255) gCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGcc < 1:2548243/45‑1 (MQ=255) tttGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATAtct < 1:1143268/51‑1 (MQ=255) tttGCTCCACCAGGGCCTGACGGCGACGCTGAAAct < 1:1131857/36‑1 (MQ=255) tttGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGa > 1:777473/1‑46 (MQ=255) ttGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTAc > 1:842593/1‑71 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGa > 1:975040/1‑44 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGa > 1:447436/1‑44 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACg > 1:1878286/1‑71 (MQ=255) ccaGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGtttttt > 1:2212908/1‑70 (MQ=255) ccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCAt < 1:2175644/49‑1 (MQ=255) caGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATAtct < 1:1627529/41‑1 (MQ=255) ccTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGttt > 1:1084801/1‑61 (MQ=255) ccTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGttt > 1:332748/1‑61 (MQ=255) cTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGtttt < 1:521581/68‑1 (MQ=255) aCGGCGACGCTGAAACTCTTGCCGGGATATctcac > 1:676847/1‑35 (MQ=255) aCGGCGACGCTGAAACTCTTGCCGGGATATctcac > 1:2525078/1‑35 (MQ=255) aCGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGt > 1:2170688/1‑55 (MQ=255) aCGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGt > 1:928121/1‑62 (MQ=255) aCGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAgtg < 1:1383562/70‑1 (MQ=255) cGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTa < 1:1722605/71‑1 (MQ=255) ggCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAac > 1:837399/1‑40 (MQ=255) tGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTAc < 1:2015519/43‑1 (MQ=255) gAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCtt < 1:302328/40‑1 (MQ=255) | CAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTA > W3110S.gb/3053406‑3053529 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |