| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,152,234 | G→A | 39.5% | D6N (GAT→AAT) | yghB → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,152,234 | 0 | G | A | 39.5% | 28.2 / 40.9 | 38 | D6N (GAT→AAT) | yghB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (9/14); new base A (7/8); total (16/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTT > W3110S.gb/3152168‑3152293 | aGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGatat < 1:2575030/71‑1 (MQ=255) cAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGc < 1:1361824/70‑1 (MQ=255) cAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCt < 1:996862/71‑1 (MQ=255) ggCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGct > 1:558894/1‑71 (MQ=255) aGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAatat < 1:1079378/56‑1 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa > 1:858547/1‑71 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCaa > 1:2109974/1‑71 (MQ=255) aaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa > 1:1302444/1‑70 (MQ=255) ggCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAacac > 1:1131563/1‑71 (MQ=255) ggCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAaca > 1:1152228/1‑70 (MQ=255) gCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGc < 1:2069144/65‑1 (MQ=255) cTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGa < 1:1727199/71‑1 (MQ=255) cTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGa < 1:212908/71‑1 (MQ=255) ttAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatcatc < 1:1747502/50‑1 (MQ=255) aaaCGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCaa > 1:1934416/1‑63 (MQ=255) aaCGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGAc < 1:2257818/68‑1 (MQ=255) gCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGcc < 1:928029/71‑1 (MQ=255) tGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAac < 1:1497261/58‑1 (MQ=255) tGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCcgc > 1:2616371/1‑71 (MQ=255) ccACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAcca > 1:1401147/1‑55 (MQ=255) ccACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTg > 1:1417768/1‑71 (MQ=255) cacaGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa < 1:2628794/52‑1 (MQ=255) cacaGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTgg < 1:845577/71‑1 (MQ=255) caGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCg > 1:2569219/1‑71 (MQ=255) aGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCACCACGACTTTGCCGCGCTGGCg > 1:555257/1‑70 (MQ=255) ggAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGa < 1:1072953/71‑1 (MQ=255) aGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGAt < 1:991034/68‑1 (MQ=255) tCCATGGCTGTTATTCAAAATATCATCGCTGCGct < 1:1288192/35‑1 (MQ=255) ccATGGCTGTTATTCAAAATATCATCGCTGCGCTCTAGCAACACGACTTTGCCGCGCTGGCGGATCCTCa < 1:1210438/70‑1 (MQ=255) cATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCata > 1:909996/1‑71 (MQ=255) aTGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCatat < 1:633580/71‑1 (MQ=255) aTGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCaa > 1:202191/1‑39 (MQ=255) tGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGa > 1:1835292/1‑61 (MQ=255) gCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGt < 1:670715/71‑1 (MQ=255) cTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGtt < 1:2120559/71‑1 (MQ=255) cTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGtt < 1:566567/71‑1 (MQ=255) ttATTCAAAATATCATCGCTGCGCTCTGGCAACACGAc < 1:420227/38‑1 (MQ=255) aTTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc > 1:1660812/1‑46 (MQ=255) | AGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTT > W3110S.gb/3152168‑3152293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |