| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,315,139 | T→A | 29.9% | D244V (GAT→GTT) | infB ← | fused protein chain initiation factor 2, IF2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,315,139 | 0 | T | A | 29.9% | 40.5 / 20.4 | 30 | D244V (GAT→GTT) | infB | fused protein chain initiation factor 2, IF2 |
| Reads supporting (aligned to +/- strand): ref base T (9/12); new base A (4/5); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGT > W3110S.gb/3315080‑3315206 | tcttcGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGtc < 1:1632008/70‑1 (MQ=255) cttcGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGtct < 1:1739585/70‑1 (MQ=255) cgcTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGc < 1:567493/71‑1 (MQ=255) ctgctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGc > 1:535065/1‑69 (MQ=255) gTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:752577/71‑1 (MQ=255) ttACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTgaga < 1:2601218/71‑1 (MQ=255) tACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCt > 1:1250529/1‑54 (MQ=255) aCGACCACGGCCAAGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCa > 1:709454/1‑61 (MQ=255) cGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAag < 1:1219682/70‑1 (MQ=255) ccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTg < 1:474280/71‑1 (MQ=255) ccacggccGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:1857743/58‑1 (MQ=255) acggccGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGAt < 1:2481978/71‑1 (MQ=255) ggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATaa > 1:2590115/1‑71 (MQ=255) ggccGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATaa < 1:1790502/71‑1 (MQ=255) ccTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGc < 1:2532166/41‑1 (MQ=255) ttCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAagt < 1:1308217/51‑1 (MQ=255) ttCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATa < 1:762512/63‑1 (MQ=255) ttCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGATgag > 1:345891/1‑47 (MQ=255) ttCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTg > 1:915651/1‑70 (MQ=255) ttCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTg > 1:1658740/1‑70 (MQ=255) tCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTgg > 1:1577870/1‑70 (MQ=255) tCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCAAGTTGAGAAGTAGTGACGTGATAATCGCTgg > 1:673507/1‑70 (MQ=255) cGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGa < 1:1759306/70‑1 (MQ=255) cGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGaa > 1:1477233/1‑71 (MQ=255) gACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAAt < 1:2329973/71‑1 (MQ=255) ttCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGaa > 1:1950752/1‑43 (MQ=255) tCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGaa > 1:1923904/1‑42 (MQ=255) cACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAAt > 1:363118/1‑66 (MQ=255) cGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGt < 1:421066/71‑1 (MQ=255) gATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGAc < 1:1215224/46‑1 (MQ=255) | TCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGT > W3110S.gb/3315080‑3315206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |