| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,688,001 | A→G | 41.5% | S440G (AGT→GGT) | yifB → | predicted bifunctional protein, enzyme and transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,688,001 | 0 | A | G | 41.5% | 17.3 / 50.2 | 41 | S440G (AGT→GGT) | yifB | predicted bifunctional protein, enzyme and transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (14/10); new base G (12/5); total (26/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGA > W3110S.gb/3687938‑3688067 | cgcgTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGa < 1:1992782/71‑1 (MQ=255) gcgTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGa > 1:2017462/1‑70 (MQ=255) aTGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACg > 1:151872/1‑71 (MQ=255) cgccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTTGATAGTCCGGaa < 1:793746/61‑1 (MQ=255) gccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATa > 1:634443/1‑63 (MQ=255) gccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAAtt > 1:886602/1‑71 (MQ=255) gccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAAtt > 1:2337252/1‑71 (MQ=255) agagagCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGaaa > 1:2392178/1‑58 (MQ=255) agagagCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCt > 1:1563629/1‑70 (MQ=255) gagCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCa > 1:318130/1‑70 (MQ=255) agCGCCAATTTAAGCGGCAGAATATACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCaaa > 1:537147/1‑71 (MQ=255) agCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCaaa < 1:2378935/71‑1 (MQ=255) agCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGaaa < 1:626241/54‑1 (MQ=255) agCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATa > 1:39484/1‑56 (MQ=255) agCGCCAATTTAAGAGGCAGAATAAACTGAATGCCTGGCTGGATa > 1:1757223/1‑45 (MQ=255) cgcCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACt > 1:1184604/1‑71 (MQ=255) gcCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCa < 1:1839329/58‑1 (MQ=255) gcCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACtt < 1:714413/71‑1 (MQ=255) aTTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:2028609/1‑69 (MQ=255) tttAAGCGGCAGAATAAACTGAATGCCTGGCTTGATAGTCCGGaaa < 1:969530/46‑1 (MQ=255) tttAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGagag < 1:1426593/71‑1 (MQ=255) cAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGc < 1:610805/71‑1 (MQ=255) aGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGc > 1:1251926/1‑70 (MQ=255) gAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGa > 1:61891/1‑71 (MQ=255) tAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGc > 1:1004063/1‑37 (MQ=255) tAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGa < 1:2250597/60‑1 (MQ=255) tAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCaa > 1:975085/1‑40 (MQ=255) tAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAAc < 1:1326692/50‑1 (MQ=255) tAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGc < 1:1270960/66‑1 (MQ=255) tAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGAtgt > 1:2205039/1‑71 (MQ=255) aaCTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTgg > 1:473079/1‑71 (MQ=255) aaCTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTgg > 1:823619/1‑71 (MQ=255) aaCTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTgg > 1:735865/1‑71 (MQ=255) tGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTg > 1:2372714/1‑71 (MQ=255) gCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACtt < 1:1093302/42‑1 (MQ=255) ccTGGCTGGATGGTCCGGAAATACTCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaagg < 1:2044397/70‑1 (MQ=255) ccTGGCTGGATAGTCCGGAAATACGCAAATTCTGCAAACTTGAGAGCGAAGATGAGATGTGGTTggaagg > 1:2160374/1‑70 (MQ=255) ctggctggATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaaggaa < 1:2258963/71‑1 (MQ=255) gctggATAGTCCGGAAATACGCCAATTCTGCAAACTTgaga > 1:1819273/1‑41 (MQ=255) tggATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACt > 1:950003/1‑70 (MQ=255) ggATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGa > 1:1579643/1‑71 (MQ=255) gATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGa > 1:637233/1‑44 (MQ=255) | CGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGA > W3110S.gb/3687938‑3688067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |