Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,326 T→C 100% pseudogene (459/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,3260TC100.0% 175.7 / NA 52pseudogene (459/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/37);  total (15/37)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGGTGCCTGCGTGGCGCTGGTAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATC  >  W3110S.gb/3824260‑3824390
                                                                  |                                                                
gggTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGa                                                              <  1:1567801/71‑1 (MQ=255)
     ccTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGaa                                                          <  1:887452/70‑1 (MQ=255)
     ccTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGa                                                           >  1:2633401/1‑69 (MQ=255)
     ccTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGa                                                           >  1:1723343/1‑69 (MQ=255)
         cGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAgg                                                       <  1:710201/69‑1 (MQ=255)
         cGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAgg                                                       <  1:1369670/69‑1 (MQ=255)
         cGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAgg                                                       <  1:2416879/69‑1 (MQ=255)
         cGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAgg                                                       <  1:2144457/69‑1 (MQ=255)
         cGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAgg                                                       <  1:1984047/69‑1 (MQ=255)
               gcTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAg                                               <  1:1364184/71‑1 (MQ=255)
               gcTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAg                                               <  1:2045921/71‑1 (MQ=255)
                 tGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGcc                                             >  1:2275795/1‑71 (MQ=255)
                    caGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGaaa                                                         >  1:2447829/2‑56 (MQ=255)
                      gATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAg                                                        <  1:2024668/55‑1 (MQ=255)
                      gATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGt                                        <  1:860196/71‑1 (MQ=255)
                       aTGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCg                                                               <  1:1370115/47‑1 (MQ=255)
                       aTGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGaa                                                          <  1:1136797/52‑1 (MQ=255)
                       aTGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGt                                        <  1:724345/70‑1 (MQ=255)
                        tGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATgg                                                  >  1:1965125/1‑59 (MQ=255)
                        tGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATgg                                                  >  1:1968363/1‑59 (MQ=255)
                        tGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGtt                                       <  1:2175888/70‑1 (MQ=255)
                        tGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGtt                                       <  1:2172757/70‑1 (MQ=255)
                         gcgcTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGt                                                 >  1:2034811/1‑59 (MQ=255)
                         gcgcTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTc                                     >  1:1737310/1‑71 (MQ=255)
                          cgcTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTc                                     <  1:2364976/70‑1 (MQ=255)
                          cgcTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCt                                    >  1:1449770/1‑71 (MQ=255)
                           gcTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATg                                                   <  1:1624442/55‑1 (MQ=255)
                             tACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGtt                                       <  1:231409/65‑1 (MQ=255)
                               cAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCgg                               <  1:1708879/71‑1 (MQ=255)
                               cAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCgg                               <  1:1926842/71‑1 (MQ=255)
                                aGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGtt                                       <  1:720409/62‑1 (MQ=255)
                                    gCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATgg                                                  >  1:1762172/1‑47 (MQ=255)
                                    gCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATgg                                                  >  1:1260839/1‑47 (MQ=255)
                                        gtggAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATgg                                                  <  1:1536235/43‑1 (MQ=255)
                                         tggAAAACGGCAAGCTGAAAACCAACCCGATGAAAgg                                                       <  1:2127654/37‑1 (MQ=255)
                                         tggAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGAt                                                    <  1:1808856/40‑1 (MQ=255)
                                          ggAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACg                    >  1:2076975/1‑71 (MQ=255)
                                                cGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAg              >  1:2116206/1‑71 (MQ=255)
                                                 ggCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATtg                          <  1:2609078/58‑1 (MQ=255)
                                                 ggCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGc             <  1:1979421/71‑1 (MQ=255)
                                                  gCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca            <  1:1287921/71‑2 (MQ=255)
                                                   cAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaa           <  1:2634669/71‑3 (MQ=255)
                                                   cAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaa           <  1:875142/71‑3 (MQ=255)
                                                   cAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaa           >  1:1312015/1‑69 (MQ=255)
                                                     aGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTc                                 <  1:1203272/47‑1 (MQ=255)
                                                     aGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTc                                 <  1:1499813/47‑1 (MQ=255)
                                                        tGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCgg                               <  1:148519/46‑1 (MQ=255)
                                                          aaaaCCAACCCGATGAAAGGGATTGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa    <  1:384074/71‑1 (MQ=255)
                                                          aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTc                                     >  1:481125/1‑38 (MQ=255)
                                                          aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGc      <  1:2029839/69‑1 (MQ=255)
                                                          aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGc      <  1:1657110/69‑1 (MQ=255)
                                                           aaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGAt   <  1:2339737/71‑1 (MQ=255)
                                                            aaccaaccCGATGAAAGGGATGGTAGCCGCAGTTTCTGTc                                 <  1:2498952/40‑1 (MQ=255)
                                                            aaccaaccCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATc  >  1:1383021/1‑71 (MQ=255)
                                                            aaccaaccCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATc  >  1:1957157/1‑71 (MQ=255)
                                                                  |                                                                
GGGTGCCTGCGTGGCGCTGGTAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATC  >  W3110S.gb/3824260‑3824390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: