| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,824,410 | T→A | 100% | pseudogene (543/687 nt) | rph → | ECK3633:JW3618:b3643; ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,824,410 | 0 | T | A | 100.0% | 112.2 / NA | 39 | pseudogene (543/687 nt) | rph | ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (22/17); total (22/17) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAGGGGACG > W3110S.gb/3824342‑3824476 | gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc > 1:967319/1‑71 (MQ=255) gTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGAc > 1:1306627/1‑71 (MQ=255) tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa > 1:782049/1‑71 (MQ=255) tCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACAt > 1:1376429/1‑70 (MQ=255) tgtaAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAg > 1:1626945/1‑71 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACAt > 1:80629/4‑62 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt > 1:1643029/4‑71 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt > 1:1669191/4‑71 (MQ=255) gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt > 1:518804/4‑71 (MQ=255) atttGCGATCTGGAATACGTTGAAGACTCAGCCGCa < 1:2021183/35‑1 (MQ=38) atttGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACgg < 1:1277048/70‑1 (MQ=255) atttGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACgg < 1:2534500/70‑1 (MQ=255) tGCGATGTGGAATACGTTGAAGACTCAGCCGCAGAGAcc > 1:6212/1‑39 (MQ=38) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgatga > 1:500806/1‑58 (MQ=255) tGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGgcg > 1:1743395/1‑71 (MQ=255) cGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGgcgc < 1:967115/70‑1 (MQ=255) ggAATACGTTGAAGACTCAGCCGCAGAGACCGACAt < 1:681280/36‑1 (MQ=255) gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGcatcat < 1:934713/68‑1 (MQ=255) gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTg < 1:1450015/70‑1 (MQ=255) gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTg < 1:585212/70‑1 (MQ=255) gAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTg > 1:283442/1‑70 (MQ=255) aaTACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGaa < 1:1627447/71‑1 (MQ=255) tACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtgatg < 1:2315738/45‑1 (MQ=255) tACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCg < 1:285062/49‑1 (MQ=255) tACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGaa < 1:2207939/51‑1 (MQ=255) tACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCAtt < 1:1695032/66‑1 (MQ=255) tACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGa < 1:221443/68‑1 (MQ=255) cGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGa > 1:2360122/1‑51 (MQ=255) cGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGttc < 1:295217/71‑3 (MQ=255) ttGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGaa < 1:2611733/47‑1 (MQ=255) ttGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGa > 1:2269440/1‑49 (MQ=255) ttGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGa > 1:2266406/1‑49 (MQ=255) tGAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGc < 1:1012708/68‑1 (MQ=255) gAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAggg > 1:2452836/1‑71 (MQ=255) gAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAggg > 1:2414950/1‑71 (MQ=255) gAAGACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAggg > 1:2393492/1‑71 (MQ=255) gAAGACTCAGCCGCAGAGACCGACATGAACGTACTGATGACCGAAGACGGGCGCATCATTGAAGTGCAgg > 1:2434719/1‑70 (MQ=255) gACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGaa > 1:579822/1‑42 (MQ=255) gACTCAGCCGCAGAGACCGACATGAACGTAGTGATGACCGaa > 1:2127389/1‑42 (MQ=255) ctcaGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAGGGGACg < 1:900491/70‑1 (MQ=255) | GTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTGATGACCGAAGACGGGCGCATCATTGAAGTGCAGGGGACG > W3110S.gb/3824342‑3824476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |