| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,824,524 | T→G | 100% | pseudogene (657/687 nt) | rph → | ECK3633:JW3618:b3643; ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,824,524 | 0 | T | G | 100.0% | 59.4 / NA | 19 | pseudogene (657/687 nt) | rph | ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (9/10); total (9/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGG‑GAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGATTTTT > W3110S.gb/3824459‑3824588 | atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTgg < 1:385963/70‑1 (MQ=255)atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc > 1:246394/1‑71 (MQ=255) aGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑gaatcga > 1:2504552/1‑66 (MQ=255) aGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCg < 1:2121168/71‑1 (MQ=255) ttCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:1070062/40‑1 (MQ=38) ccATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑GGGTGAATCGAATCCATTg < 1:1948866/56‑1 (MQ=255) aTGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑gaatcga > 1:1215668/1‑46 (MQ=255) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATcc < 1:2002050/49‑1 (MQ=255) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTg < 1:2331062/53‑1 (MQ=255) gAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGAAggcggc > 1:676895/1‑71 (MQ=255) cTCACCTTGTTGGCGCTGGCCCGAGGGG‑gaatcgaatc < 1:572548/38‑1 (MQ=21) cTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTg < 1:542296/43‑1 (MQ=25) accTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTg > 1:2249652/3‑63 (MQ=255) accTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACt > 1:2272440/3‑70 (MQ=255) ccTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCa < 1:611850/64‑1 (MQ=255) ccTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGa > 1:1909900/2‑71 (MQ=255) ttgttgGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCaaa > 1:1530667/1‑65 (MQ=255) ttgGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGAttttt < 1:1466265/71‑1 (MQ=255) tgGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGAc > 1:1825581/1‑40 (MQ=37) | ATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGG‑GAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGATTTTT > W3110S.gb/3824459‑3824588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |