| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,246,990 | C→T | 26.7% | R40H (CGT→CAT) | malG ← | maltose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,246,990 | 0 | C | T | 26.7% | 48.6 / 18.2 | 30 | R40H (CGT→CAT) | malG | maltose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (8/14); new base T (2/6); total (10/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
AACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGC > W3110S.gb/4246921‑4247055 | aaCGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACg > 1:468587/1‑71 (MQ=255) aGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCga < 1:2518867/71‑1 (MQ=255) tttCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCgaga < 1:1265696/71‑1 (MQ=255) tCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGAt < 1:1209827/70‑1 (MQ=255) tCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGAt < 1:2321284/70‑1 (MQ=255) ccAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCgaga > 1:2054819/1‑68 (MQ=255) aGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcg < 1:384882/71‑1 (MQ=255) ccAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGAcc < 1:1037010/70‑1 (MQ=255) agaTTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATcag < 1:1162956/70‑1 (MQ=255) aTTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGc < 1:208865/56‑1 (MQ=255) ttGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCg > 1:982866/1‑71 (MQ=255) tCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGaa > 1:1392053/1‑71 (MQ=255) aGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGc < 1:247197/71‑1 (MQ=255) aGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcga > 1:1230080/1‑43 (MQ=255) cGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATa < 1:1744130/71‑1 (MQ=255) aaaGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagt > 1:190692/1‑70 (MQ=255) aaaGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagt > 1:2474882/1‑70 (MQ=255) aaaGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAag > 1:1264952/1‑69 (MQ=255) aaGTTTCCCTGATGCAGCAAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAag < 1:1243608/68‑1 (MQ=255) aGTTTCCCTGATGCAGCGAGATAGCGACGACCATc < 1:729341/35‑1 (MQ=255) aGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagc < 1:1962800/71‑1 (MQ=255) aGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtag > 1:1653771/1‑70 (MQ=255) aGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagc < 1:2013413/71‑1 (MQ=255) gTTTCCCTGACGCAGCGAGATAGCGACGACCATcagcag < 1:2232245/39‑1 (MQ=255) gTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtag > 1:1792545/1‑69 (MQ=255) tttCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACAt < 1:2275005/47‑1 (MQ=255) tttCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaaa < 1:2016869/63‑1 (MQ=255) tCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACAt < 1:157237/45‑1 (MQ=255) ccTGACGCAGCGAGATAGCGACGA‑CATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcagtagc < 1:2004544/70‑1 (MQ=255) cTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa < 1:1479973/57‑1 (MQ=255) | AACGCCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGC > W3110S.gb/4246921‑4247055 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |