| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,340,671 | G→A | 33.4% | T347T (ACC→ACT) | adiC ← | arginine:agmatin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,340,671 | 0 | G | A | 33.4% | 49.4 / 36.5 | 43 | T347T (ACC→ACT) | adiC | arginine:agmatin |
| Reads supporting (aligned to +/- strand): ref base G (16/12); new base A (7/7); total (24/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01 | |||||||||||
CGCACAGGTGTAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACCGACGATAATCAGCCCC > W3110S.gb/4340602‑4340737 | cgcACAGGTGTAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTg < 1:316529/70‑1 (MQ=255) tgtAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGttt < 1:1014717/71‑1 (MQ=255) tAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTgg < 1:780633/71‑1 (MQ=255) aaGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGt > 1:2194673/1‑70 (MQ=255) aaGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGt > 1:153088/1‑70 (MQ=255) aGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGa < 1:1028986/71‑1 (MQ=255) aGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGa < 1:1340201/71‑1 (MQ=255) atatGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGaaa > 1:1264809/1‑71 (MQ=255) tGGCACCAGTGTAAAGATGACCGAGCCGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAAtg > 1:1969201/1‑70 (MQ=255) tGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAAtgc < 1:1263611/71‑1 (MQ=255) aCCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATtctgct < 1:2470252/71‑1 (MQ=255) aGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAAtg > 1:1026917/1‑63 (MQ=255) gtgtAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGc > 1:1734696/1‑71 (MQ=255) tgtAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGa < 1:964166/68‑1 (MQ=255) gtAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTg > 1:1923604/1‑71 (MQ=255) gtAAAGATGACCGAGACGGAAGAAACAAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTg > 1:1327322/1‑71 (MQ=255) tAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCAGAGCTg > 1:3415/1‑70 (MQ=255) aaaGATGACCGAGACGGAAGAACCCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTgg > 1:1187781/1‑70 (MQ=255) gACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATgg > 1:866383/1‑71 (MQ=255) gACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATgg > 1:2033261/1‑71 (MQ=255) gagaCGGAAGAAACCAGACCGAACTCTTTCGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGtcat > 1:2600580/1‑71 (MQ=255) aCGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGa > 1:800094/1‑41 (MQ=255) aCGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGtcatca > 1:931182/1‑70 (MQ=255) aCGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCaa < 1:1536209/71‑1 (MQ=255) aCGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCaa < 1:1303231/71‑1 (MQ=255) ggAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTg > 1:985015/1‑38 (MQ=255) ggAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTg > 1:364706/1‑38 (MQ=255) aagaaACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAAtg < 1:2262936/41‑1 (MQ=255) aagaaACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAAtg < 1:1772857/41‑1 (MQ=255) gaaACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCaaaa > 1:1312726/1‑67 (MQ=255) aaaCCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGa < 1:282431/54‑1 (MQ=255) aCCAGACCGAACTCTTTGGTCGCGTTTGGTGAAAtgc > 1:1791516/1‑37 (MQ=255) ccAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAg < 1:1656146/50‑1 (MQ=255) aGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACcga > 1:2296659/1‑67 (MQ=255) aGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACCGACGAt < 1:1722735/71‑1 (MQ=255) gACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCaaaa > 1:209444/1‑60 (MQ=255) gACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACCGACGATa < 1:1241903/71‑1 (MQ=255) ccGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACCGACGATaa < 1:2219928/70‑1 (MQ=255) aaCTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACCGACGATAAt < 1:1630982/68‑1 (MQ=255) tctTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACCGACGATa < 1:1744379/63‑1 (MQ=255) tttGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCaaaa > 1:2559155/1‑50 (MQ=255) tttGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACCGACGATAATCAGcccc > 1:879335/1‑70 (MQ=255) tGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAg > 1:2125989/1‑35 (MQ=255) tGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATgg < 1:2534948/39‑1 (MQ=255) | CGCACAGGTGTAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACCGACGATAATCAGCCCC > W3110S.gb/4340602‑4340737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |