| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,416,062 | A→G | 25.8% | N27D (AAT→GAT) | yjfK → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,416,062 | 0 | A | G | 25.8% | 50.2 / 17.9 | 31 | N27D (AAT→GAT) | yjfK | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (12/11); new base G (5/3); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCG > W3110S.gb/4416005‑4416130 | cTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATa > 1:1948603/1‑61 (MQ=255) tGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTa > 1:695072/1‑69 (MQ=255) tGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTa > 1:625253/1‑69 (MQ=255) tttGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTa > 1:2063590/1‑67 (MQ=255) tttGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTa < 1:524708/67‑1 (MQ=255) tttGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTAc < 1:2313264/68‑1 (MQ=255) gCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATa < 1:1033880/54‑1 (MQ=255) aaGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCaa < 1:1531189/50‑1 (MQ=255) taaGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGcgt > 1:1759041/1‑71 (MQ=255) ccATCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCt < 1:2458457/46‑1 (MQ=255) cAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTc < 1:1848464/53‑1 (MQ=255) gCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGcgtttcgttt < 1:482342/71‑1 (MQ=255) tATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGc > 1:696672/1‑71 (MQ=255) gCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTgg > 1:2110407/1‑70 (MQ=255) cTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTc > 1:1694785/1‑44 (MQ=255) cTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTg > 1:2187814/1‑68 (MQ=255) cGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAAtt < 1:1978512/70‑1 (MQ=255) cTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGaa > 1:1174170/1‑66 (MQ=255) cTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGaa > 1:1100478/1‑66 (MQ=255) cTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCt < 1:1323653/71‑1 (MQ=255) ggACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTg < 1:1152141/69‑1 (MQ=255) aCTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTgg > 1:42314/1‑53 (MQ=255) aCTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTg < 1:2334385/71‑1 (MQ=255) ttCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAAt < 1:555666/60‑1 (MQ=255) cATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGaa > 1:958999/1‑51 (MQ=255) cATCTCAATAGTGGCTTTACGCTCGACACGTTAGcgtttcg < 1:1009104/41‑1 (MQ=255) cATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTg > 1:1829299/1‑45 (MQ=255) cATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTg > 1:1571989/1‑45 (MQ=255) tctcGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGc < 1:1081264/44‑1 (MQ=255) tctcAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCt < 1:1197752/60‑1 (MQ=255) ctcAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGcc > 1:1709798/1‑71 (MQ=255) tcAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCg > 1:411876/1‑71 (MQ=255) aaTAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATg > 1:2108220/1‑49 (MQ=255) | CTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCG > W3110S.gb/4416005‑4416130 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |