| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,434,349 | T→A | 21.4% | intergenic (+112/‑197) | fklB → / → cycA | FKBP‑type peptidyl‑prolyl cis‑trans isomerase/D‑alanine/D‑serine/glycine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,434,349 | 0 | T | A | 21.4% | 54.8 / 11.9 | 28 | intergenic (+112/‑197) | fklB/cycA | FKBP‑type peptidyl‑prolyl cis‑trans isomerase/D‑alanine/D‑serine/glycine transporter |
| Reads supporting (aligned to +/- strand): ref base T (12/10); new base A (3/3); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
CAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTA > W3110S.gb/4434281‑4434418 | cAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTAttttt > 1:604557/1‑71 (MQ=255) gCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTtgt > 1:1871114/1‑71 (MQ=255) aTAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAg > 1:507080/1‑71 (MQ=255) aaCCCTTAAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTAtttt < 1:1104514/62‑1 (MQ=255) aCCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGt < 1:1041932/71‑1 (MQ=255) cAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTcgcg > 1:1515151/1‑71 (MQ=255) cAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTcgc < 1:169568/70‑1 (MQ=255) gTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGtt < 1:485850/71‑1 (MQ=255) gTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGtt < 1:293427/71‑1 (MQ=255) gTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGtt < 1:823371/71‑1 (MQ=255) cAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTc > 1:1888602/1‑60 (MQ=255) cGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGa > 1:2050747/1‑71 (MQ=255) ttttGTGTTGCAAAGACCCCGTAAGCGTGTAtttt < 1:1178495/35‑1 (MQ=255) ttttGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGatat < 1:2406764/66‑1 (MQ=255) ttttGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGacac < 1:471202/71‑1 (MQ=255) ttttGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGa > 1:2574623/1‑68 (MQ=255) tttGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGt < 1:1442938/60‑1 (MQ=255) ttGTGTTGCAAAGACCCCGTAAGCGTGTATTTTtg > 1:2467055/1‑35 (MQ=255) gtgtTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcactcac > 1:247149/1‑71 (MQ=255) ttGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGatat < 1:1512786/59‑1 (MQ=255) gacccccGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACAt < 1:1462060/68‑1 (MQ=255) cGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAAt > 1:169214/1‑70 (MQ=255) gTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGata > 1:678242/1‑45 (MQ=255) tAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACttt < 1:1493369/57‑1 (MQ=255) tAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTa < 1:2465079/58‑1 (MQ=255) aaGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGat > 1:2227568/1‑42 (MQ=255) aGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTaa > 1:2406386/1‑57 (MQ=255) aGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACa > 1:379350/1‑71 (MQ=255) aGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATaaaa > 1:1140418/1‑65 (MQ=255) tgtATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAg < 1:569946/71‑1 (MQ=255) tattatTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACAt > 1:971937/1‑66 (MQ=255) tATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACAt > 1:100912/1‑66 (MQ=255) attattGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGAt > 1:1046547/1‑70 (MQ=255) tttttGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact < 1:2591829/41‑1 (MQ=255) tttttGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact < 1:148710/41‑1 (MQ=255) tttGTGAGCTGTTTCGCGTTATCACCGTGATATGaca > 1:2294556/1‑37 (MQ=255) tttGTGAGCTGTTTCGCGTTATCACCGTGATATGaca > 1:1632613/1‑37 (MQ=255) tttGTGAGCTGTTTCGCGTTATCACCGTGATATGAcactca > 1:499422/1‑41 (MQ=255) tttGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTa > 1:685436/1‑70 (MQ=255) | CAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTA > W3110S.gb/4434281‑4434418 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |