| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,563,589 | T→A | 100% | K207M (AAG→ATG) | iadA ← | isoaspartyl dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,563,589 | 0 | T | A | 95.6% | 100.1 / ‑5.4 | 36 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base A (14/20); total (14/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563522‑4563654 | ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:2126652/71‑1 (MQ=255) tCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa < 1:2484687/71‑1 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt < 1:2253625/71‑1 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt < 1:631252/71‑1 (MQ=255) cagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:1367518/63‑1 (MQ=255) gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt < 1:706203/69‑1 (MQ=255) gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt < 1:1304334/69‑1 (MQ=255) gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt < 1:1771361/69‑1 (MQ=255) gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt < 1:1728372/69‑1 (MQ=255) cTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgt < 1:2436069/70‑1 (MQ=255) cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:1044200/1‑71 (MQ=255) ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca < 1:1024239/70‑1 (MQ=255) ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca < 1:1988151/70‑1 (MQ=255) caTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:2275255/70‑1 (MQ=255) aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCg < 1:2295708/52‑1 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatt > 1:1018278/1‑40 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatt > 1:1181180/1‑40 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt > 1:768467/1‑45 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:608525/1‑71 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:1826603/1‑71 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:885926/1‑71 (MQ=255) gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:1429336/50‑1 (MQ=255) ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca < 1:246899/58‑1 (MQ=255) cagcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTa < 1:1695016/70‑1 (MQ=255) gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:101829/38‑1 (MQ=255) gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:1050559/38‑1 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgt > 1:225917/1‑39 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc > 1:1405207/1‑63 (MQ=255) aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg < 1:1533905/37‑1 (MQ=255) tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:1489097/47‑1 (MQ=255) aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCa > 1:1198263/1‑52 (MQ=255) tAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGcc < 1:326126/49‑1 (MQ=255) gTAACGCCTTTTTACTGTCGCCCATGTGTAACACGGTGACGCCAGGtt < 1:90074/48‑1 (MQ=255) gTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:1297308/37‑1 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt > 1:1904189/1‑46 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTa > 1:2066834/1‑47 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTa > 1:1432404/1‑47 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAcc > 1:2354304/1‑61 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg > 1:1426065/1‑71 (MQ=255) gCCATTTTACTGTCGCCCAGGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaaa > 1:1666608/1‑57 (MQ=255) | GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563522‑4563654 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |