| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,154 | C→T | 89.8% | D606D (GAC→GAT) | thrA → | fused aspartokinase I and homoserine dehydrogenase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,154 | 0 | C | T | 89.8% | 98.4 / 4.9 | 39 | D606D (GAC→GAT) | thrA | fused aspartokinase I and homoserine dehydrogenase I |
| Reads supporting (aligned to +/- strand): ref base C (2/2); new base T (12/23); total (14/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAAT > W3110S.gb/2086‑2221 | tcgtcgATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGatac > 1:932613/1‑71 (MQ=255) tGGATTACTACCATCTGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGtt < 1:2031130/71‑1 (MQ=255) tactacCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGc > 1:2553549/1‑71 (MQ=255) tactacCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGc > 1:728067/1‑71 (MQ=255) tactacCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGc > 1:2008039/1‑71 (MQ=255) aTCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTa > 1:2494946/1‑71 (MQ=255) aGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTgg > 1:147933/1‑58 (MQ=255) aGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTgg > 1:1489457/1‑58 (MQ=255) aGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTgg > 1:1584193/1‑58 (MQ=255) aTGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTAtt > 1:202657/1‑68 (MQ=255) tGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTgag < 1:332350/70‑1 (MQ=255) aaaTCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTgaga < 1:1628583/61‑1 (MQ=255) aaaTCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTgaga < 1:1083176/61‑1 (MQ=255) aaaTCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTgaga < 1:267187/61‑1 (MQ=255) aaTCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTgaga < 1:1408259/60‑1 (MQ=255) tCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGa > 1:65494/1‑42 (MQ=255) tCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGa > 1:1890258/1‑42 (MQ=255) ggcgTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTc < 1:1945072/71‑1 (MQ=255) cgTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTgaga < 1:571550/52‑1 (MQ=255) tAAATTCCTCTATGATACCATCGTTGGGGCTGGATTAcc < 1:703279/39‑1 (MQ=38) tAAATTCCTCTATGATACCAACGTTGGGGCTGGATTAcc < 1:712070/39‑1 (MQ=255) tAAATTCCTCTATGATACCAACGTTGGGGCTGGATTAcc < 1:1226238/39‑1 (MQ=255) tAAATTCCTCTATGATACCAACGTTGGGGCTGGATTAcc < 1:534148/39‑1 (MQ=255) aaTTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAAt > 1:1830145/1‑68 (MQ=255) aaTTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATg > 1:532831/1‑69 (MQ=255) aaTTCCTCTATGATACAAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATg > 1:421283/1‑69 (MQ=255) ccTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGt < 1:821094/70‑1 (MQ=255) ccTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGt < 1:2399871/70‑1 (MQ=255) ccTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGt < 1:1958602/70‑1 (MQ=255) ctctATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGtg < 1:1216489/70‑1 (MQ=255) tGATACCAACGTTGGGGCTGGATTACCGGTTATTTAGAACCTGCAAAATCTGCTCAATGc < 1:83424/60‑1 (MQ=255) tGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTGAATGc < 1:1125440/60‑1 (MQ=255) tGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGc < 1:312187/60‑1 (MQ=255) tGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGc < 1:173858/60‑1 (MQ=255) tGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGtgatg < 1:293822/68‑1 (MQ=255) tGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGtgatg < 1:2590468/68‑1 (MQ=255) tGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGtgatg < 1:1315476/68‑1 (MQ=255) tGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGtgatg < 1:842643/68‑1 (MQ=255) tGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAAt > 1:1842235/1‑71 (MQ=255) tGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGtgatg < 1:1656013/68‑1 (MQ=255) | TCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAAT > W3110S.gb/2086‑2221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |