| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 907,713 | G→A | 100% | T331I (ACC→ATC) | ybjT ← | conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 907,713 | 0 | G | A | 75.0% | 32.1 / 7.7 | 20 | T331I (ACC→ATC) | ybjT | conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (5/0); new base A (8/7); total (13/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAA > W3110S.gb/907662‑907764 | aTTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATa < 1:1513329/54‑1 (MQ=255) cACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAAc > 1:969825/1‑71 (MQ=255) cACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAAc > 1:2455512/1‑71 (MQ=255) cACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAAc > 1:2396306/1‑71 (MQ=255) tACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATa < 1:1473092/43‑1 (MQ=255) tACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAAt < 1:1435956/65‑1 (MQ=255) tACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAAt < 1:37523/65‑1 (MQ=255) aCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTa > 1:533854/1‑71 (MQ=255) aCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTa > 1:1250561/1‑71 (MQ=255) cTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACt > 1:2454961/1‑71 (MQ=255) cTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACt > 1:374457/1‑71 (MQ=255) cTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACt > 1:1377931/1‑71 (MQ=255) gCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTcc > 1:1651015/1‑71 (MQ=255) gCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTcc > 1:1448944/1‑71 (MQ=255) aaGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACACCGCCTg > 1:1383299/1‑43 (MQ=255) aaGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCggg > 1:760631/1‑71 (MQ=255) ggCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCa < 1:1758223/71‑1 (MQ=255) ggCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCa < 1:814250/71‑1 (MQ=255) gCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCaa < 1:480307/71‑1 (MQ=255) tttAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGt > 1:250316/1‑46 (MQ=255) | ATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAA > W3110S.gb/907662‑907764 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |