Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,151,925	A→C	100%	P207P (CCA→CCC)	fabD →	malonyl‑CoA‑[acyl‑carrier‑protein] transacylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,151,925	0	A	C	100.0%	42.5 / NA	14	P207P (CCA→CCC)	fabD	malonyl‑CoA‑[acyl‑carrier‑protein] transacylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/8);  total (6/8)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGCGGGCGCAAAACGCGCGCTGCCGTTACCAGTGAGCGTACCGTCTCACTGTGCGCTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACC  >  W3110S.gb/1151859‑1151975                                                                   |                                                   ggcgggcgCAAAACGCGCGCTGCCGTTACCAGTGAGCGTACCGTCTCACTGTGCGCTGATGAAACcagcag                                                <  1:722520/71‑1 (MQ=255)       cgcAAAACGCGCGCTGCCGTTACCAGTGAGCGTACCGTCTCACTGTGCGCTGATTAAACCCGCAGCCGAc                                           >  1:410179/1‑70 (MQ=255)       cgcAAAACGCGCGCTGCCGTTACCAGTGAGCGTACCGTCTCACTGTGCGCTGATGAAACCCGCAGCCGAc                                           >  1:2656791/1‑70 (MQ=255)             aCGCGCGCTGCCGTTACCAGTGAGCGTACCGTCTCACTGTGCGCTGATGAAACCCGCAGCCGACAAACTg                                     <  1:2406399/70‑1 (MQ=255)                                    gCGTACCGTCTCACTGTGCGCTGATGAAACCCGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCAcc             <  1:226511/71‑1 (MQ=255)                                    gCGTACCGTCTCACTGTGCGCTGATGAAACCCGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCAcc             <  1:2438790/71‑1 (MQ=255)                                    gCGTACCGTCTCACTGTGCGCTGATGAAACCCGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCAcc             <  1:244247/71‑1 (MQ=255)                                              tcACTGTGCGCTGATGAAACCCGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCa    >  1:2425627/1‑70 (MQ=255)                                              tcACTGTGCGCTGATGAAACCCGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCAc   >  1:1551553/1‑71 (MQ=255)                                              tcACTGTGCGCTGATGAAACCCGCAGCCGACAAACTGGCAGTAGAATTAGCCAAAATCACCTTTAACGCa    >  1:1191783/1‑70 (MQ=255)                                                aCTGTGCGCTGATGAAACCCGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCAcc  >  1:1478251/1‑70 (MQ=255)                                                 cTGTGCGCTGATGAAACCCGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCttt          <  1:1322913/61‑1 (MQ=255)                                                 cTGTGCGCTGATGAAACCCGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCttt          <  1:1486843/61‑1 (MQ=255)                                                 cTGTGCGCTGATGAAACCCGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCttt          <  1:1550556/61‑1 (MQ=255)                                                 cTGTGCGCTGATGAAACCCGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCttt          <  1:2027554/61‑1 (MQ=255)                                                                   |                                                   GGCGGGCGCAAAACGCGCGCTGCCGTTACCAGTGAGCGTACCGTCTCACTGTGCGCTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACC  >  W3110S.gb/1151859‑1151975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑