Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,303,631 C→T 100% Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,303,6310CT100.0% 72.3 / NA 27Q246Y (CAG→TAT) oppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/18);  total (9/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAG  >  W3110S.gb/1303568‑1303686
                                                               |                                                       
aacgaactAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                  >  1:1306910/1‑71 (MQ=255)
aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                  >  1:2251321/1‑71 (MQ=255)
aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                  >  1:1957371/1‑71 (MQ=255)
  cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                  >  1:2475181/1‑69 (MQ=255)
            gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                       <  1:1096077/70‑1 (MQ=255)
                  gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                                   >  1:2052679/1‑52 (MQ=255)
                  gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt                                  <  1:1783864/69‑1 (MQ=255)
                  gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                >  1:1759881/1‑71 (MQ=255)
                    aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                              <  1:968698/71‑1 (MQ=255)
                    aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                              <  1:66139/71‑1 (MQ=255)
                    aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                              <  1:523894/71‑1 (MQ=255)
                    aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                              <  1:334890/71‑1 (MQ=255)
                     cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                             <  1:900062/71‑1 (MQ=255)
                     cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                             <  1:2658027/71‑1 (MQ=255)
                            cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                       >  1:152305/1‑54 (MQ=255)
                            cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                      <  1:1902187/71‑1 (MQ=255)
                            cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                      <  1:293277/71‑1 (MQ=255)
                                cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                         <  1:262141/48‑1 (MQ=39)
                                cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                    <  1:1529876/69‑1 (MQ=255)
                                 tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                         <  1:2649572/47‑1 (MQ=39)
                                 tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                         <  1:453272/47‑1 (MQ=39)
                                 tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                         <  1:1760352/47‑1 (MQ=39)
                                      gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                       >  1:142390/1‑44 (MQ=38)
                                        acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                      <  1:633443/59‑1 (MQ=255)
                                             gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg     >  1:332943/1‑71 (MQ=255)
                                                 aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAg  <  1:2223968/70‑1 (MQ=255)
                                                 aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAg  <  1:2142079/70‑1 (MQ=255)
                                                               |                                                       
AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAG  >  W3110S.gb/1303568‑1303686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: