| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,303,633 | G→T | 100% | Q246Y (CAG→TAT) | oppA → | oligopeptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,303,633 | 0 | G | T | 100.0% | 72.9 / NA | 27 | Q246Y (CAG→TAT) | oppA | oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (9/18); total (9/18) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAG > W3110S.gb/1303568‑1303686 | aacgaactAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc > 1:1306910/1‑71 (MQ=255)aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc > 1:2251321/1‑71 (MQ=255)aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc > 1:1957371/1‑71 (MQ=255) cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc > 1:2475181/1‑69 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa < 1:1096077/70‑1 (MQ=255) gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa > 1:2052679/1‑52 (MQ=255) gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt < 1:1783864/69‑1 (MQ=255) gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc > 1:1759881/1‑71 (MQ=255) aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg < 1:968698/71‑1 (MQ=255) aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg < 1:66139/71‑1 (MQ=255) aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg < 1:523894/71‑1 (MQ=255) aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg < 1:334890/71‑1 (MQ=255) cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:900062/71‑1 (MQ=255) cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:2658027/71‑1 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa > 1:152305/1‑54 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:1902187/71‑1 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:293277/71‑1 (MQ=255) cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:262141/48‑1 (MQ=39) cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa < 1:1529876/69‑1 (MQ=255) tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:2649572/47‑1 (MQ=39) tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:453272/47‑1 (MQ=39) tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:1760352/47‑1 (MQ=39) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa > 1:142390/1‑44 (MQ=38) acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:633443/59‑1 (MQ=255) gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg > 1:332943/1‑71 (MQ=255) aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAg < 1:2223968/70‑1 (MQ=255) aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAg < 1:2142079/70‑1 (MQ=255) | AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAG > W3110S.gb/1303568‑1303686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |