| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,413,585 | C→T | 8.3% | V26V (GTG→GTA) | ydaO ← | predicted C32 tRNA thiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,413,585 | 0 | C | T | 8.3% | 181.1 / 6.3 | 60 | V26V (GTG→GTA) | ydaO | predicted C32 tRNA thiolase |
| Reads supporting (aligned to +/- strand): ref base C (28/26); new base T (2/3); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01 | |||||||||||
GAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTG > W3110S.gb/1413523‑1413647 | gagaGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTac > 1:511075/1‑70 (MQ=255)gagaGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTac > 1:1229049/1‑70 (MQ=255)gagaGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTac > 1:595685/1‑70 (MQ=255)gagaGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTac > 1:535618/1‑70 (MQ=255) ccATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGt > 1:966376/1‑71 (MQ=255) ccATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGt > 1:1283939/1‑71 (MQ=255) atgatgCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGttt > 1:2135406/1‑71 (MQ=255) atgatgCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCAACGTTACGACGAAGACGttt > 1:1580248/1‑71 (MQ=255) tgCGATCGCCTTCTTCTATCATATTGAAGTCAGCAATGGCTTCGCCTACGTTa > 1:2017206/1‑53 (MQ=255) tgCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCTACGTTa > 1:528872/1‑53 (MQ=255) aTCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAAt < 1:57508/70‑1 (MQ=255) aTCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAAt < 1:866514/70‑1 (MQ=255) ttcttcAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGtt > 1:2163668/1‑69 (MQ=255) cttcAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAgg > 1:1291366/1‑71 (MQ=255) cttcAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAgg > 1:868185/1‑71 (MQ=255) cttcAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAgg > 1:395315/1‑71 (MQ=255) cttcAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAgg > 1:2385578/1‑71 (MQ=255) cttcAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAg < 1:513769/70‑1 (MQ=255) cAATCATTTTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAgg < 1:557548/68‑1 (MQ=255) cAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAAt < 1:2291947/59‑1 (MQ=255) cAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTg > 1:332267/1‑71 (MQ=255) cAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTg > 1:99134/1‑71 (MQ=255) cAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTg > 1:1008852/1‑71 (MQ=255) aaTCATATTGAAGTCAGCAATGGCTTCGCCTACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGt < 1:229051/71‑1 (MQ=255) aaTCATATTGAAGTCAGCAATGGCTTCGCCTACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGt < 1:1570840/71‑1 (MQ=255) aaTCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTg < 1:526011/70‑1 (MQ=255) aTCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTa < 1:306963/71‑1 (MQ=255) tCATATTGAAGTCAGCAATGGCTTCGCCTACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGt < 1:38767/69‑1 (MQ=255) tCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAg > 1:718727/1‑64 (MQ=255) tCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAg > 1:1505532/1‑64 (MQ=255) tCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAg > 1:1607870/1‑64 (MQ=255) ttGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGttc > 1:1761149/1‑71 (MQ=255) ttGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGttc > 1:1662792/1‑71 (MQ=255) tCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGt < 1:854688/42‑1 (MQ=255) tCAGCAATGGCTCCGCCCACGTTACGACGCAGACGTTTTTGt < 1:1874834/42‑1 (MQ=255) cAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAAt < 1:2364950/44‑1 (MQ=255) gCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTg < 1:1953828/54‑1 (MQ=255) gCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTg < 1:1142413/54‑1 (MQ=255) gCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTg < 1:1331940/54‑1 (MQ=255) gCAATGGCTT‑TCCCACGTTACGACGCAGACGTTTTTGTAAttt > 1:1471283/1‑43 (MQ=37) ctaTGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTATTTTGTTCAGGTTg < 1:1238530/51‑1 (MQ=255) cAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTg < 1:2650113/44‑1 (MQ=255) aaTGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTa > 1:2146746/1‑54 (MQ=255) aaTGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTa > 1:114482/1‑54 (MQ=255) aaTGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTaa < 1:1417975/71‑1 (MQ=255) aaTGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTaa < 1:2073719/71‑1 (MQ=255) aTGGCTTCGCCCACTTTACGACGCAGACGTTTTTGTAATTTGtt < 1:2283003/44‑1 (MQ=255) aTGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGtt < 1:1008019/44‑1 (MQ=255) aTGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGtt < 1:2544373/44‑1 (MQ=255) aTGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGtt < 1:1628671/44‑1 (MQ=255) aTGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGtt < 1:668697/44‑1 (MQ=255) aTGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGt < 1:2228349/57‑1 (MQ=255) aTGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAAt < 1:1772125/71‑1 (MQ=255) aTGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAAt < 1:1985650/71‑1 (MQ=255) aTGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAAt < 1:2298006/71‑1 (MQ=255) gCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATttg > 1:633307/1‑71 (MQ=255) gCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATttg > 1:115865/1‑71 (MQ=255) gCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATttg > 1:834448/1‑71 (MQ=255) gCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATttg > 1:613367/1‑71 (MQ=255) gCTTAGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATttg > 1:1523484/1‑71 (MQ=255) | GAGAGGCAAACCATGATGCGATCGCCTTCTTCAATCATATTGAAGTCAGCAATGGCTTCGCCCACGTTACGACGCAGACGTTTTTGTAATTTGTTCAGGTTGTATTGTTCTTTCTTTGTAATTTG > W3110S.gb/1413523‑1413647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |