| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,691,177 | G→A | 14.3% | A1008V (GCG→GTG) | purL ← | phosphoribosylformyl‑glycineamide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,691,177 | 0 | G | A | 14.3% | 71.0 / 3.8 | 28 | A1008V (GCG→GTG) | purL | phosphoribosylformyl‑glycineamide synthetase |
| Reads supporting (aligned to +/- strand): ref base G (14/9); new base A (2/2); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTTGATATCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGC > W3110S.gb/2691121‑2691244 | cGTTGATATCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTc > 1:545294/1‑67 (MQ=255)cGTTGATATCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCt > 1:1039280/1‑69 (MQ=255)cGTTGATATCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCt > 1:291661/1‑69 (MQ=255)cGTTGATATCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCt > 1:1371887/1‑69 (MQ=255)cGTTGATATCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCt > 1:2564232/1‑69 (MQ=255)cGTTGATATCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTg < 1:2507447/70‑1 (MQ=255)cGTTGATATCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTg < 1:744636/70‑1 (MQ=255) tatCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCt < 1:1037078/54‑1 (MQ=255) tatCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTg < 1:1217272/64‑1 (MQ=255) tCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGAt > 1:232016/1‑64 (MQ=255) tCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGAt > 1:2393698/1‑64 (MQ=255) gAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGCTCGGcaca > 1:2308740/1‑69 (MQ=255) gAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGcaca > 1:1247210/1‑69 (MQ=255) gAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGcac > 1:1300725/1‑68 (MQ=255) gAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGcac > 1:334640/1‑68 (MQ=255) gAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACt > 1:550683/1‑71 (MQ=255) gAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACt > 1:409088/1‑71 (MQ=255) ttttACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCgg < 1:875954/56‑1 (MQ=255) ttCAGGCCCGGATCGGCGTCGTTAGATTTCACCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCa < 1:2091247/70‑1 (MQ=255) ttCAGGCCCGGATCGGCGTCGTTAGATTTCACCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCa < 1:1616025/70‑1 (MQ=255) cAGGCCCGGATCGGCGTCGTTAGATTTCACCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAgg > 1:987958/1‑70 (MQ=255) cAGGACCGGATCGGCGTCGTTAGATTTCACCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAg > 1:708285/1‑69 (MQ=255) ccGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGc < 1:1573312/71‑1 (MQ=255) aTCGGCGTCGTTAGATTTCTCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCt < 1:629949/71‑1 (MQ=255) gtcgtTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAgg < 1:2129193/71‑1 (MQ=255) ttAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACg < 1:541746/47‑1 (MQ=255) aGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGtt < 1:2163538/70‑1 (MQ=255) ttCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGc > 1:83234/1‑71 (MQ=255) | CGTTGATATCGAACGACAGTTTTACATTCAGGCCCGGATCGGCGTCGTTAGATTTCGCCTGATGCTCCTGATCGGCACACTCCGGGTTGTCACGCAGGCGCTGCATCTGCCAGGTAGTTTCTGC > W3110S.gb/2691121‑2691244 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |