| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,866,110 | T→A | 100% | intergenic (‑20/+160) | rpoS ← / ← nlpD | RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,866,110 | 0 | T | A | 100.0% | 161.0 / NA | 54 | intergenic (‑20/+160) | rpoS/nlpD | RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (20/34); total (20/34) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTC > W3110S.gb/2866043‑2866171 | cAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:2353315/1‑71 (MQ=255)cAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:213356/1‑71 (MQ=255)cAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:595530/1‑71 (MQ=255)cAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAt > 1:303122/1‑70 (MQ=255) cGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTaa < 1:1667421/71‑1 (MQ=255) tgtgtgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAg > 1:1153396/1‑69 (MQ=255) gCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa > 1:998985/1‑70 (MQ=255) gCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa > 1:836780/1‑70 (MQ=255) cGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaaa < 1:2325807/70‑1 (MQ=255) aTAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:731090/50‑1 (MQ=255) aaCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaa > 1:1545122/1‑64 (MQ=255) aaCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaa > 1:2659468/1‑64 (MQ=255) aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTc > 1:815296/1‑70 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTaa > 1:2129409/1‑43 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTaa > 1:928111/1‑43 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTaa > 1:2113731/1‑43 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTct < 1:2339579/36‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTct < 1:372303/36‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTct < 1:2635560/36‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTct < 1:375475/36‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTct < 1:1017814/36‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:683466/35‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:1348490/35‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:271144/35‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:2413699/35‑1 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:178983/1‑35 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:356894/1‑35 (MQ=255) cGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc < 1:247179/70‑1 (MQ=255) cGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtct < 1:1049508/69‑1 (MQ=255) cGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtct < 1:951859/69‑1 (MQ=255) cGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtct < 1:1211810/69‑1 (MQ=255) cGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtct < 1:1347267/69‑1 (MQ=255) cGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtct < 1:1467241/69‑1 (MQ=255) cGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtca > 1:1377644/1‑71 (MQ=255) cGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtca > 1:774933/1‑71 (MQ=255) ccAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtcatca < 1:695082/71‑1 (MQ=255) aaaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCaaa > 1:2080923/1‑71 (MQ=255) aaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCa < 1:1591563/44‑1 (MQ=255) aaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCa < 1:1815207/44‑1 (MQ=255) tCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTc > 1:2492851/1‑71 (MQ=255) tCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTc > 1:2391538/1‑71 (MQ=255) ttATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtcatca < 1:218087/63‑1 (MQ=255) ttATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtcatca < 1:2192041/63‑1 (MQ=255) ttATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGc < 1:718284/71‑1 (MQ=255) ttATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGc < 1:144562/71‑1 (MQ=255) ttATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGc < 1:840474/71‑1 (MQ=255) ttATCACTGGGTTCCAATTCTACTAAGGCCTTTTCCTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGc < 1:1702366/71‑1 (MQ=255) tATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGc < 1:2605940/70‑1 (MQ=255) cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc < 1:1705674/71‑1 (MQ=255) cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc < 1:673996/71‑1 (MQ=255) cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc < 1:2115218/71‑1 (MQ=255) cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc < 1:844582/71‑1 (MQ=255) cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc < 1:2619531/71‑1 (MQ=255) tCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtcatca < 1:1423892/51‑1 (MQ=255) | CAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTC > W3110S.gb/2866043‑2866171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |