| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,152,234 | G→A | 73.9% | D6N (GAT→AAT) | yghB → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,152,234 | 0 | G | A | 73.9% | 75.6 / 31.0 | 46 | D6N (GAT→AAT) | yghB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (7/5); new base A (17/17); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
CAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGT > W3110S.gb/3152167‑3152298 | cAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAat < 1:1777889/70‑1 (MQ=255)cAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAat < 1:693653/70‑1 (MQ=255) tAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATatcatc > 1:1864516/1‑65 (MQ=255) ggCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAat < 1:2211714/57‑1 (MQ=255) aTAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAata < 1:2062639/53‑1 (MQ=255) aTAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAata < 1:2280443/53‑1 (MQ=255) aTAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCt < 1:1847958/61‑1 (MQ=255) aTAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCt < 1:1473031/61‑1 (MQ=255) aTAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCt < 1:1088327/61‑1 (MQ=255) aTAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCt < 1:893399/61‑1 (MQ=255) tAAAGGCTTAAACTCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCa < 1:118399/71‑1 (MQ=255) tAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCa < 1:1590517/71‑1 (MQ=255) tAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGACa < 1:785040/71‑1 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGa > 1:2300290/1‑49 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGa > 1:1722914/1‑49 (MQ=255) aaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa > 1:1369333/1‑70 (MQ=255) aaaCGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGAc < 1:37235/69‑1 (MQ=255) aaCGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACttt > 1:2088219/1‑71 (MQ=255) cGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGc > 1:187884/1‑71 (MQ=255) cGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGc > 1:1522528/1‑71 (MQ=255) ttCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTgcgc < 1:2263626/47‑1 (MQ=255) ttCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc > 1:2080852/1‑71 (MQ=255) ttCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc > 1:140250/1‑71 (MQ=255) ccACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTg > 1:847663/1‑71 (MQ=255) caGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACttt < 1:1560751/59‑1 (MQ=255) caGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCg < 1:850243/71‑1 (MQ=255) caGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCg < 1:764758/71‑1 (MQ=255) caGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCg < 1:2643110/71‑1 (MQ=255) caGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCg < 1:2618284/71‑1 (MQ=255) ggAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGc > 1:1818009/1‑59 (MQ=255) gAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGAt > 1:1388583/1‑71 (MQ=255) aaaGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATc > 1:2023255/1‑71 (MQ=255) aaGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACtt > 1:1837255/1‑53 (MQ=255) aaGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACtt > 1:1188235/1‑53 (MQ=255) aaGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACtt > 1:1275225/1‑53 (MQ=255) aaGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACtt > 1:1517016/1‑53 (MQ=255) aaGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACtt > 1:1791962/1‑53 (MQ=255) gCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGt < 1:1038498/71‑1 (MQ=255) cTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGtt < 1:1001012/71‑1 (MQ=255) gTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAg > 1:1974587/1‑71 (MQ=255) aTTCAAAATATCATCGCTGCGCTCTGGCTACACGACTTTGCCGCGCTGGCGGATCCTCATATTGtt < 1:1001016/66‑1 (MQ=255) aTTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCata > 1:2134064/1‑61 (MQ=255) aTTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgt > 1:2366859/1‑71 (MQ=255) aTTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgt > 1:473383/1‑71 (MQ=255) aTTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgt > 1:706479/1‑71 (MQ=255) aTTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgt > 1:176053/1‑71 (MQ=255) | CAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGT > W3110S.gb/3152167‑3152298 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |