| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,315,139 | T→A | 100% | D244V (GAT→GTT) | infB ← | fused protein chain initiation factor 2, IF2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,315,139 | 0 | T | A | 80.2% | 48.1 / 6.8 | 25 | D244V (GAT→GTT) | infB | fused protein chain initiation factor 2, IF2 |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base A (9/11); total (9/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGG > W3110S.gb/3315073‑3315209 | ttgCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCTCGGCCGCCTTCGACTTCACGAACGc < 1:532834/70‑1 (MQ=255)ttgCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCt < 1:1539533/71‑1 (MQ=255) cTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCg < 1:1922642/71‑1 (MQ=255) cttcGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGtct < 1:674747/70‑1 (MQ=255) cGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCtt > 1:1199733/1‑61 (MQ=255) cGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTg > 1:395626/1‑71 (MQ=255) cGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTg > 1:2240543/1‑71 (MQ=255) gcgcTGCTTTGGCGTTACGTCCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTgg < 1:219485/71‑1 (MQ=255) gcgcTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTgg < 1:163849/71‑1 (MQ=255) cgcTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTgg < 1:991933/70‑1 (MQ=255) gctgctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCg < 1:1108618/71‑1 (MQ=255) gctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAg > 1:2271052/1‑70 (MQ=255) gctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAg > 1:819247/1‑70 (MQ=255) aCGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAag > 1:19774/1‑71 (MQ=255) gACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAagta < 1:2658023/71‑1 (MQ=255) cacggccacggcCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTg < 1:2386078/71‑1 (MQ=255) ggccacggccGCCTTCGACTTCACGAACGCTTTCGTCTTCTGc < 1:261321/43‑1 (MQ=255) acggccGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTg > 1:2279642/1‑54 (MQ=255) cgccTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCg < 1:2000355/71‑1 (MQ=255) cgccTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCg < 1:577164/71‑1 (MQ=255) gccTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCg < 1:2026938/70‑1 (MQ=255) ccTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCg < 1:166837/38‑1 (MQ=255) cACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCtt > 1:2320614/1‑69 (MQ=255) cACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCtt > 1:1215595/1‑69 (MQ=255) cGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGt < 1:754041/71‑1 (MQ=255) tCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCgg > 1:2159363/1‑71 (MQ=255) | TTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGG > W3110S.gb/3315073‑3315209 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |