Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,374 C→T 100% pseudogene (507/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,3740CT100.0% 85.2 / NA 31pseudogene (507/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/20);  total (11/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGT  >  W3110S.gb/3824308‑3824436
                                                                  |                                                              
cGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAg                                                            >  1:1567859/1‑71 (MQ=255)
cGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAg                                                            >  1:759081/1‑71 (MQ=255)
   cAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGcaa                                                         <  1:2081560/71‑3 (MQ=255)
           aaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGAt                                                 <  1:2394451/71‑1 (MQ=255)
               cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                             <  1:739129/71‑1 (MQ=255)
                      aTGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                          <  1:1374735/51‑2 (MQ=255)
                      aTGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                          <  1:887256/51‑2 (MQ=255)
                      aTGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                          <  1:424417/51‑2 (MQ=255)
                         aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                          <  1:2493548/48‑2 (MQ=255)
                         aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                    <  1:98981/70‑1 (MQ=255)
                         aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                    <  1:2329738/70‑1 (MQ=255)
                           aGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATATGCGATCTGGAATACGTTGAAg                                 <  1:1683518/71‑1 (MQ=255)
                                tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                    <  1:1758700/63‑1 (MQ=255)
                                tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                    <  1:2628388/63‑1 (MQ=255)
                                tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                    <  1:1885686/63‑1 (MQ=255)
                                tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGActca                            <  1:2489363/71‑2 (MQ=255)
                                 ggTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGActca                            <  1:887380/70‑2 (MQ=255)
                                  gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                  <  1:1756343/63‑1 (MQ=255)
                                  gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                  <  1:1245086/63‑1 (MQ=255)
                                  gTAGCCCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                  <  1:1088448/62‑1 (MQ=255)
                                   tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACg                                       >  1:1718350/1‑57 (MQ=255)
                                   tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACg                                       >  1:1611925/1‑57 (MQ=255)
                                   tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACg                                       >  1:1329422/1‑57 (MQ=255)
                                     gCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg                        <  1:2147498/70‑1 (MQ=255)
                                            tttCTGTCTGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCag                     <  1:2460481/66‑1 (MQ=255)
                                              tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCagag                   >  1:1864132/1‑66 (MQ=255)
                                                      taTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg      >  1:2190348/2‑71 (MQ=255)
                                                        ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg      >  1:773132/1‑69 (MQ=255)
                                                          gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt  >  1:2339685/4‑71 (MQ=255)
                                                          gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt  >  1:39173/4‑71 (MQ=255)
                                                          gtaaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt  >  1:1045375/4‑71 (MQ=255)
                                                                  |                                                              
CGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGT  >  W3110S.gb/3824308‑3824436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: