| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,824,524 | T→G | 100% | pseudogene (657/687 nt) | rph → | ECK3633:JW3618:b3643; ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,824,524 | 0 | T | G | 100.0% | 61.4 / NA | 19 | pseudogene (657/687 nt) | rph | ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (7/12); total (7/12) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGG‑GAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGATTTTT > W3110S.gb/3824457‑3824588 | tcatTGAAGCGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTg < 1:1652209/71‑1 (MQ=255) cAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑ggg‑gg < 1:2112123/71‑2 (MQ=255) gCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGct < 1:1945420/48‑1 (MQ=255) gCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑gaatcgaatc > 1:1300405/1‑71 (MQ=255) cAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g > 1:2429069/1‑57 (MQ=255) aGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑GGGTGAATCGAATCCATTg > 1:2041341/1‑71 (MQ=255) ggCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGt < 1:347950/71‑1 (MQ=255) ggCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGt < 1:2533899/71‑1 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAg < 1:192656/70‑1 (MQ=255) aTGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGAAggcg > 1:1658272/1‑71 (MQ=255) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g < 1:748659/35‑1 (MQ=37) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g < 1:2054062/35‑1 (MQ=37) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g < 1:2168904/35‑1 (MQ=37) tCACCTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCa < 1:1784528/68‑1 (MQ=255) accTTGTTGGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAAc > 1:911657/3‑69 (MQ=255) tgttgGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCa < 1:1307179/46‑1 (MQ=255) tgttgGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGAtt > 1:2036974/1‑70 (MQ=255) tgttgGCGCTGGCCCGA‑GGGTGAATCGAATCCATTGTAGCGACGCa < 1:695269/46‑1 (MQ=255) ttgGCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGAttttt < 1:2197652/71‑1 (MQ=255) ggCGCTGGCCCGAGGGG‑GAATCGAATCCATTGTAGCGACGCAGaa > 1:1973719/1‑45 (MQ=255) | TCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGG‑GAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGATTTTT > W3110S.gb/3824457‑3824588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |