| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,090,233 | G→A | 25.0% | E763E (GAG→GAA) | malP → | maltodextrin phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,090,233 | 0 | G | A | 25.0% | 33.4 / 8.9 | 20 | E763E (GAG→GAA) | malP | maltodextrin phosphorylase |
| Reads supporting (aligned to +/- strand): ref base G (9/6); new base A (2/3); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01 | |||||||||||
TGGCGGATTTCGCAGCCTATGTAGAGGCACAAAAGCAGGTGGATGTGCTGTACCGCGACCAGGAGGCCTGGACTCGCGCGGCGATCCTCAATACCGCCCGCTGCGGTATGTTTAGCTCGGATCGCTC > W3110S.gb/4090169‑4090295 | tGGCGGATTTCGCAGCCTATGTAGAGGCACAAAAGCAGGTGGATGTGCTGTACCGCGACCAGGAAGcc < 1:782225/68‑1 (MQ=255)tGGCGGATTTCGCAGCCTATGTAGAGGCACAAAAGCAGGTGGATGTGCTGTACCGCGACCAGGAAGcc < 1:2680097/68‑1 (MQ=255)tGGCGGATTTCGCAGCCTATGTAGAGGCACAAAAGCAGGTGGATGTGCTGTACCGCGACCAGGAAGcc < 1:2326844/68‑1 (MQ=255) gCGGATTTCGCAGCCTATGTAGAGGCACAAAAGCAGGTGGATGTGCTGTACCGCGACCAGGAAGCCTg > 1:1164096/1‑68 (MQ=255) gCGGATTTCGCAGCCTATGTAGAGGCACAAAAGCAGGTGGATGTGCTGTACCGCGACCAGGAAGCCTg > 1:2650183/1‑68 (MQ=255) cGGATTTCGCAGCCTATGTAGAGGCACAAAAGCAGGTGGATGTGCTGTACCGCGACCAGGAGGCCTGGACt < 1:165608/71‑1 (MQ=255) cGGATTTCGCAGCCTATGTAGAGGCACAAAAGCAGGTGGATGTGCTGTACCGCGACCAGGAGGCCTGGACt < 1:2605828/71‑1 (MQ=255) cGGATTTCGCAGCCTATGTAGAGGCACAAAAGCAGGTGGATGTGCTGTACCGCGACCAGGAGGCCTGGACt < 1:1073783/71‑1 (MQ=255) tAGAGGCACAAAAGCAGGTGGATGTGCTGTACCGCGACCAGGAGGCCTGGACTCGCGCGGCGATCCTCa > 1:152243/1‑69 (MQ=255) gagGCACAAAAGCAGGTGGATGTGCTGTACCGCGACCAGGAGGCCTGGACTCGCGCGGCGATCCTCAATAc > 1:1909863/1‑71 (MQ=255) gCACAAAAGCAGGTGGATGTGCTGTACCGCGACCAGGAGGCCTGGACTCGCGCGGCGATCCTCAATAccgc > 1:2506699/1‑71 (MQ=255) gCACAAAAGCAGGTGGATGTGCTGTACCGCGACCAGGAGGCCTGGACTCGCGCGGCGATCCTCAATAcagc > 1:679650/1‑71 (MQ=255) gATATGCTGTACCGCGACCAGGAGGCCTGGACTCGCGCGGCGATCCTc < 1:2447686/48‑1 (MQ=255) tgtgCTGTACCGCGACCAGGAGGCCTGGACTCGCGCGGCGATCCTCAATACCGCCCGCTGCGGTATGtt > 1:1521599/1‑69 (MQ=255) tgtgCTGTACCGCGACCAGGAGGCCTGGACTCGCGCGGCGATCCTCAATACCGCCCGCTGCGGTATGtt > 1:328789/1‑69 (MQ=255) tgtgCTGTACCGCGACCAGGAGGCCTGGACTCGCGCGGCGATCCTCAATACCGCCCGCTGCGGTATGtt > 1:551814/1‑69 (MQ=255) aCCGCGACCAGGAGGCCTGGACTCGCGCGGCGATCCTCAATACCGCCCGCTGCGGTATGTTTAGCTCGGAt > 1:1783602/1‑71 (MQ=255) aCCGCGACCAGGAGGCCTGGACTCGCGCGGCGATCCTCAATACCGCCCGCTGCGGTATGTTTAGCTCGGAt > 1:1279723/1‑71 (MQ=255) cgcgACCAGGAGGCCTGGACTCGCGCGGCGATCCTCAATACCGCCCGCTGCGGTATGTTTAGCTCGGAt < 1:2670955/69‑1 (MQ=255) gACCAGGAGGCCTGGACTCGCGCGGCGATCCTCAATACCGCCCGCTGCGGTATGTTTAGCTCGGATCGctc < 1:1569277/71‑1 (MQ=255) | TGGCGGATTTCGCAGCCTATGTAGAGGCACAAAAGCAGGTGGATGTGCTGTACCGCGACCAGGAGGCCTGGACTCGCGCGGCGATCCTCAATACCGCCCGCTGCGGTATGTTTAGCTCGGATCGCTC > W3110S.gb/4090169‑4090295 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |