| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,246,990 | C→T | 77.3% | R40H (CGT→CAT) | malG ← | maltose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,246,990 | 0 | C | T | 77.3% | 81.7 / 24.2 | 44 | R40H (CGT→CAT) | malG | maltose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (3/7); new base T (16/18); total (19/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
CCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCA > W3110S.gb/4246925‑4247056 | ccAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGc > 1:8796/1‑71 (MQ=255) gTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCgag < 1:2666897/71‑1 (MQ=255) ttCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCgaga > 1:539712/1‑70 (MQ=255) tCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCgaga > 1:1222983/1‑69 (MQ=255) tCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCgaga > 1:1226273/1‑69 (MQ=255) tCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCgaga > 1:2069457/1‑69 (MQ=255) tGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGGGAGATAGcgac > 1:262404/1‑71 (MQ=255) aTCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGcgacga > 1:821609/1‑71 (MQ=255) tCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCgacgac < 1:2142185/71‑1 (MQ=255) gaTTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATcag > 1:2095669/1‑69 (MQ=255) gaTTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATcag > 1:2513743/1‑69 (MQ=255) cTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCggg < 1:2672617/70‑1 (MQ=255) cTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGa < 1:954956/71‑1 (MQ=255) gggATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGaa < 1:1903320/68‑1 (MQ=255) gATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATaa < 1:1471228/71‑1 (MQ=255) gATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATaa < 1:2451907/71‑1 (MQ=255) aTCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAAt > 1:2318130/1‑71 (MQ=255) aGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCg < 1:2299738/70‑1 (MQ=255) aGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCg < 1:22050/70‑1 (MQ=255) gCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAAt > 1:447134/1‑66 (MQ=255) cTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGc < 1:249472/71‑1 (MQ=255) cTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGc < 1:1879015/71‑1 (MQ=255) tCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa < 1:905750/70‑1 (MQ=255) tCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa < 1:137456/70‑1 (MQ=255) tCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGc < 1:563706/64‑1 (MQ=255) gCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa > 1:1299127/1‑68 (MQ=255) gCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa > 1:702959/1‑68 (MQ=255) gCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa > 1:1598664/1‑68 (MQ=255) gCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa > 1:1329317/1‑68 (MQ=255) gCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa > 1:1068595/1‑68 (MQ=255) cAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATa < 1:246574/53‑1 (MQ=255) cAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATa < 1:306366/53‑1 (MQ=255) cAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa < 1:1940843/67‑1 (MQ=255) cAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa < 1:1376507/67‑1 (MQ=255) aGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATaa > 1:1439755/1‑51 (MQ=255) aGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATaa > 1:1024404/1‑51 (MQ=255) gTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGc < 1:435479/54‑1 (MQ=255) gTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGc < 1:2058467/54‑1 (MQ=255) tCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcag > 1:2144951/1‑69 (MQ=255) cTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAgtagcagtagca < 1:1721557/71‑1 (MQ=255) cTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAgtagcagtagca < 1:2417358/71‑1 (MQ=255) cTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAgtagcagtagca < 1:1024174/71‑1 (MQ=255) cTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAgtagcagtagca < 1:2187942/71‑1 (MQ=255) cTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTTCGATAAAAAgtagcagtagca < 1:2426953/71‑1 (MQ=255) | CCAGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCA > W3110S.gb/4246925‑4247056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |