Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	4,340,671	G→A	65.9%	T347T (ACC→ACT)	adiC ←	arginine:agmatin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,340,671	0	G	A	65.9%	45.7 / 41.3	44	T347T (ACC→ACT)	adiC	arginine:agmatin
Reads supporting (aligned to +/- strand):  ref base G (6/9);  new base A (14/15);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACAGGTGTAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACCGACGAT  >  W3110S.gb/4340604‑4340727                                                                    |                                                         cacaGGTGTAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTc                                                       >  1:860339/1‑71 (MQ=255)    aGGTGTAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTcgcg                                                    <  1:11166/71‑1 (MQ=255)        gtAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTg                                                >  1:2659859/1‑71 (MQ=255)           aaGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGt                                              >  1:2377813/1‑70 (MQ=255)           aaGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACAGAACTCTTTGGTCGCGTTTg                                                >  1:1680545/1‑68 (MQ=255)              atatGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGt                                              <  1:212279/67‑1 (MQ=255)              atatGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGt                                              <  1:2474162/67‑1 (MQ=255)              atatGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGt                                              <  1:348026/67‑1 (MQ=255)              atatGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGt                                              <  1:1475813/67‑1 (MQ=255)                        aGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAg                                >  1:774448/1‑71 (MQ=255)                         gtgtAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGaa                                           <  1:2642317/59‑1 (MQ=255)                         gtgtAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGaa                                           <  1:2606314/59‑1 (MQ=255)                         gtgtAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGaa                                           <  1:1006843/59‑1 (MQ=255)                          tgtAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGtt                                                  <  1:866702/51‑1 (MQ=255)                          tgtAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGtt                                                  <  1:1002721/51‑1 (MQ=255)                          tgtAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGtta                                            <  1:1660631/57‑3 (MQ=255)                          tgtAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGa                                            <  1:1541889/57‑1 (MQ=255)                           gtAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAAt                                         >  1:163567/1‑59 (MQ=255)                            tAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGa                                            <  1:272601/55‑1 (MQ=255)                            tAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAAt                                         >  1:1739582/1‑58 (MQ=255)                            tAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTg                             >  1:71280/1‑70 (MQ=255)                            tAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTg                             >  1:791270/1‑70 (MQ=255)                            tAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTg                             >  1:377872/1‑70 (MQ=255)                              aaGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGaa                          <  1:1799186/71‑1 (MQ=255)                              aaGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTg                                                >  1:2039453/1‑49 (MQ=255)                              aaGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTg                                                >  1:1645728/1‑49 (MQ=255)                              aaGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTg                                             >  1:582744/1‑52 (MQ=255)                              aaGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGaa                                           >  1:1412872/1‑54 (MQ=255)                              aaGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTgg                            >  1:1889856/1‑69 (MQ=255)                              aaGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTgg                            >  1:506651/1‑69 (MQ=255)                                 aTGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAAtgctg                                     >  1:653376/1‑57 (MQ=255)                                  tGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATg                      <  1:48785/71‑1 (MQ=255)                                  tGACCGAGACGGAAGAAAACAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATg                      <  1:970260/71‑1 (MQ=255)                                          actgaagaaACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCaa              <  1:267535/68‑1 (MQ=255)                                          aCGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCaa              <  1:1575351/71‑1 (MQ=255)                                            ggAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCaaaa            <  1:2333267/71‑1 (MQ=255)                                            ggAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCaaaa            <  1:951676/71‑1 (MQ=255)                                              aagaaACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATa          >  1:2427392/1‑71 (MQ=255)                                              aagaaACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGATGCTGAGCTGGAAGATGGTCATCAAAATa          >  1:1115521/1‑71 (MQ=255)                                                      aGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCaaaa            >  1:1440233/1‑61 (MQ=255)                                                      aGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACCGACGAt  <  1:1029961/71‑1 (MQ=255)                                                               tctTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAg                         <  1:1768916/39‑1 (MQ=255)                                                               tctTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAg                         <  1:1569946/39‑1 (MQ=255)                                                               tctTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAg                         <  1:1475024/39‑1 (MQ=255)                                                                    |                                                         CACAGGTGTAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACCGACGAT  >  W3110S.gb/4340604‑4340727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑