| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,416,062 | A→G | 100% | N27D (AAT→GAT) | yjfK → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,416,062 | 0 | A | G | 91.5% | 142.6 / 3.9 | 47 | N27D (AAT→GAT) | yjfK | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (1/3); new base G (17/26); total (18/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTG > W3110S.gb/4416002‑4416127 | cGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCtt < 1:1036333/71‑1 (MQ=255) gTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCtt < 1:329698/70‑1 (MQ=255) gATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGAt < 1:1259083/48‑1 (MQ=255) taaGCCGGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGc > 1:1909189/1‑69 (MQ=255) taaGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGcgt > 1:442824/1‑71 (MQ=255) taaGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGcgt > 1:1604491/1‑71 (MQ=255) taaGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGcgt > 1:677609/1‑71 (MQ=255) taaGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGcg > 1:654539/1‑70 (MQ=255) gCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGAAACGCTAAcgtgtc < 1:2310285/71‑1 (MQ=255) tATCGCTCGTTGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGcgtttc < 1:1451146/65‑1 (MQ=255) tATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGcgtttc < 1:84709/65‑1 (MQ=255) tATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGcgtttc < 1:1980553/65‑1 (MQ=255) tCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCttt < 1:683852/41‑1 (MQ=255) cGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGcgtttc < 1:1832085/62‑1 (MQ=255) gCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTAc > 1:1278518/1‑41 (MQ=255) gCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTAc > 1:512068/1‑41 (MQ=255) gCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTg < 1:1484774/66‑1 (MQ=255) gCTCGTGGGCCGCTGGGACTTCATCTCGATAGTGGCTTTAc > 1:2436449/1‑41 (MQ=255) tCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGc > 1:44723/1‑65 (MQ=255) tCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCt > 1:767448/1‑66 (MQ=255) tCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCt > 1:2074738/1‑66 (MQ=255) gTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGaa < 1:2379987/68‑1 (MQ=255) tGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATg < 1:2118348/71‑1 (MQ=255) tGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATg < 1:588294/71‑1 (MQ=255) tGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATg < 1:1220864/71‑1 (MQ=255) tGGTCCGCCGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATg < 1:778450/71‑1 (MQ=255) ggTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGa > 1:546063/1‑68 (MQ=255) ggTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATg > 1:369114/1‑70 (MQ=255) gTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGaa < 1:1714017/71‑1 (MQ=255) gTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGaa < 1:136636/71‑1 (MQ=255) gTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGaa < 1:44386/71‑1 (MQ=255) cGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGt > 1:2222859/1‑42 (MQ=255) cGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGt > 1:2640052/1‑42 (MQ=255) cGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGt > 1:1752487/1‑42 (MQ=255) cGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGt > 1:1333854/1‑42 (MQ=255) cTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCt < 1:273254/71‑1 (MQ=255) cTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCt < 1:1997678/71‑1 (MQ=255) cTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCt < 1:687688/71‑1 (MQ=255) tGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTa < 1:156571/41‑1 (MQ=255) ggACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGGAGATGAATTGCTg < 1:604993/69‑1 (MQ=255) ggACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTg < 1:1001888/69‑1 (MQ=255) gACTTCATCTCGATAGTGTCTTTACGCTCGACACGTTAGcgtttc < 1:90934/45‑1 (MQ=255) ttCATCTCGATAGTGGCTTTACGCTCGACACGTTAGcgtttc < 1:1777325/42‑1 (MQ=255) ttCATCTCGATAGTGGCTTTACGCTCGACACGTTAGcgtttc < 1:1277842/42‑1 (MQ=255) ttCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATg < 1:2661918/57‑1 (MQ=255) aTCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCt < 1:2189508/70‑1 (MQ=255) aTCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTg > 1:2127156/1‑71 (MQ=255) | CGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTG > W3110S.gb/4416002‑4416127 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |