| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,434,349 | T→A | 100% | intergenic (+112/‑197) | fklB → / → cycA | FKBP‑type peptidyl‑prolyl cis‑trans isomerase/D‑alanine/D‑serine/glycine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,434,349 | 0 | T | A | 84.8% | 76.5 / 8.6 | 33 | intergenic (+112/‑197) | fklB/cycA | FKBP‑type peptidyl‑prolyl cis‑trans isomerase/D‑alanine/D‑serine/glycine transporter |
| Reads supporting (aligned to +/- strand): ref base T (4/1); new base A (16/12); total (20/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACAT > W3110S.gb/4434289‑4434410 | aaCCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTg > 1:2334574/1‑71 (MQ=255)aaCCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTg > 1:2153581/1‑71 (MQ=255) aCCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGt < 1:1293384/71‑1 (MQ=255) gTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGtt < 1:1478354/71‑1 (MQ=255) ttGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTAt > 1:1145807/1‑71 (MQ=255) ttGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTAt > 1:805661/1‑71 (MQ=255) tGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTAt < 1:2671359/70‑1 (MQ=255) aaCGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGt < 1:2008613/71‑1 (MQ=255) cGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGa < 1:1165740/71‑1 (MQ=255) cTATTTTGTGTTGCAAAGACCCCGTAATCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGa < 1:78610/71‑1 (MQ=255) ttttGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGtt < 1:479200/53‑1 (MQ=255) ttttGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGa < 1:841568/63‑1 (MQ=255) tttGTGTT‑CAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:797347/1‑70 (MQ=255) ttGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTAt > 1:2593026/1‑53 (MQ=255) ttGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:2142139/1‑70 (MQ=255) ttGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:230582/1‑70 (MQ=255) ttGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact < 1:818147/70‑1 (MQ=255) ttGTGTTGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:1223368/1‑70 (MQ=255) ttGCAAAGACCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGt < 1:987396/45‑1 (MQ=255) aaGACCCCGTAAGCGTGTATTATTGTGAGCTTTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACa < 1:561080/71‑1 (MQ=255) aCCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATaa < 1:1691974/71‑1 (MQ=255) cccGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATc > 1:2436367/1‑39 (MQ=255) cccGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATc > 1:2180297/1‑39 (MQ=255) cccGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATc > 1:1847933/1‑39 (MQ=255) aGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACa > 1:2474049/1‑71 (MQ=255) aGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACa > 1:487786/1‑71 (MQ=255) aGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACa > 1:1437760/1‑71 (MQ=255) aGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACa > 1:1144009/1‑71 (MQ=255) aGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACa > 1:427095/1‑71 (MQ=255) aGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACa > 1:687752/1‑71 (MQ=255) aGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACa > 1:1716569/1‑71 (MQ=255) aGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACa > 1:1379942/1‑71 (MQ=255) aGCGTGTA‑TATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACAt < 1:2389722/71‑1 (MQ=255) tattatTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACAt > 1:2673495/1‑66 (MQ=255) tattatTGTGAGCTGTTTCGCGTTATCACAGTGATATGACACTCACTTTAAACATAAAATTAACAt > 1:2456617/1‑66 (MQ=255) | AACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACAT > W3110S.gb/4434289‑4434410 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |