| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,154 | C→T | 75.0% | D606D (GAC→GAT) | thrA → | fused aspartokinase I and homoserine dehydrogenase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,154 | 0 | C | T | 75.0% | 31.6 / 9.6 | 20 | D606D (GAC→GAT) | thrA | fused aspartokinase I and homoserine dehydrogenase I |
| Reads supporting (aligned to +/- strand): ref base C (3/2); new base T (6/9); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
GATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATT > W3110S.gb/2095‑2222 | gATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTgg > 1:665104/1‑71 (MQ=255)gATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTgg > 1:1512551/1‑71 (MQ=255) ctacCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTg < 1:1435546/71‑1 (MQ=255) ctacCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTg < 1:1834774/71‑1 (MQ=255) cAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTAcc > 1:189678/1‑71 (MQ=255) aGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCg > 1:1172617/1‑71 (MQ=255) aGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCg > 1:521977/1‑71 (MQ=255) tATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTAtt > 1:1375774/1‑69 (MQ=255) aaaaTCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCaaa < 1:1518702/71‑1 (MQ=255) aaaaTCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCaaa < 1:660349/71‑1 (MQ=255) aTCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATc > 1:1650143/1‑71 (MQ=255) gcggcgTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTg < 1:909005/70‑1 (MQ=255) aaaTTCCTCTATGATACCAACGTTGGGGCTGGATTAcc < 1:1683971/38‑1 (MQ=255) aaaTTCCTCTATGATACCAACGTTGGGGCTGGATTAcc < 1:351168/38‑1 (MQ=255) aaaTTCCTCTATGATACCAACGTTGGGGCTGGATTAcc < 1:543038/38‑1 (MQ=255) aaTTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCa < 1:354368/71‑1 (MQ=255) aaTTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATCGAGAACCTGCAAAATCTg > 1:294662/1‑62 (MQ=255) aaTTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATCGAGAACCTGCAAAATCTg > 1:820216/1‑62 (MQ=255) ccTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGt < 1:597958/70‑1 (MQ=255) ccTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGt < 1:1832822/70‑1 (MQ=255) acacCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAAtt < 1:940051/70‑1 (MQ=255) | GATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATT > W3110S.gb/2095‑2222 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |