| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 713,690 | G→A | 10.0% | L94L (CTG→CTA) | seqA → | regulatory protein for replication initiation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 713,690 | 0 | G | A | 10.0% | 183.4 / 9.3 | 60 | L94L (CTG→CTA) | seqA | regulatory protein for replication initiation |
| Reads supporting (aligned to +/- strand): ref base G (22/32); new base A (3/3); total (25/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
GCGTGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGAATCGTTGCACGGTCGTACAC > W3110S.gb/713624‑713760 | gCGTGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCtgttgt > 1:1691132/1‑71 (MQ=255)gCGTGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCtgttgt > 1:482381/1‑71 (MQ=255) cGTGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTc > 1:1693871/1‑71 (MQ=255) cGTGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTc > 1:1627325/1‑71 (MQ=255) cGTGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTc > 1:1620705/1‑71 (MQ=255) cGTGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTc > 1:809060/1‑71 (MQ=255) cGTGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTc > 1:1717100/1‑71 (MQ=255) cGTGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTc > 1:900135/1‑71 (MQ=255) cGTGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTc > 1:195166/1‑71 (MQ=255) cGTGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTc > 1:1381066/1‑71 (MQ=255) tGAACTTCTGCTTTCGGATGAATTCGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTa < 1:1064849/71‑1 (MQ=255) tGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTa < 1:1233456/71‑1 (MQ=255) tGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTa < 1:736023/71‑1 (MQ=255) tGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTa < 1:166744/71‑1 (MQ=255) tGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTa < 1:779114/71‑1 (MQ=255) aaCTTCTGCTTTCGGATGAATACGCAGAGCAAAGGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTaca > 1:1812856/1‑71 (MQ=255) aaCTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTaca > 1:1532612/1‑71 (MQ=255) aaCTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTaca > 1:1539935/1‑71 (MQ=255) aaCTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTaca > 1:619017/1‑71 (MQ=255) aaCTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTaca > 1:1157192/1‑71 (MQ=255) aaCTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTaca > 1:682023/1‑71 (MQ=255) aaCTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTaca > 1:1397169/1‑71 (MQ=255) cTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACtat < 1:1616534/70‑1 (MQ=255) cTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTATTGTCTACACtata < 1:1571492/68‑1 (MQ=255) ggATGTATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTATTGTCTACACTATATTCTCtt < 1:1751642/70‑1 (MQ=255) ggATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTATTGTCTACACTATATTCTCtt < 1:1689173/70‑1 (MQ=255) gAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGc > 1:192164/1‑71 (MQ=255) gAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGc > 1:1708766/1‑71 (MQ=255) tACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCtt < 1:610356/63‑1 (MQ=255) tACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCtt < 1:1198491/63‑1 (MQ=255) tACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCtt < 1:1803716/63‑1 (MQ=255) tACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGa > 1:36098/1‑65 (MQ=255) tACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGa > 1:615601/1‑65 (MQ=255) cGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAg > 1:897176/1‑70 (MQ=255) gCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCtt < 1:1616871/60‑1 (MQ=255) cAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCg < 1:1537950/71‑1 (MQ=255) cAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCg < 1:52812/71‑1 (MQ=255) cAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCg < 1:808014/71‑1 (MQ=255) cAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCAACACTATATTCTCTTGACGCCCAGGCg < 1:1417733/71‑1 (MQ=255) agagCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCg < 1:1127087/70‑1 (MQ=255) aaaGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACta < 1:816325/43‑1 (MQ=255) cgagcgGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGc < 1:1531972/71‑1 (MQ=255) cgagcgGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGc < 1:1783647/71‑1 (MQ=255) cgagcgGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGc < 1:1099146/71‑1 (MQ=255) gagcgGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGc < 1:319443/70‑1 (MQ=255) ggTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACgg < 1:986574/71‑1 (MQ=255) gTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCtt < 1:1594077/42‑1 (MQ=255) gTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGa < 1:43605/71‑1 (MQ=255) gTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGa < 1:490819/71‑1 (MQ=255) gTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGa < 1:578736/71‑1 (MQ=255) gTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGa < 1:218128/71‑1 (MQ=255) tCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCtt < 1:402453/41‑1 (MQ=255) tCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGa < 1:341115/70‑1 (MQ=255) cAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGAAt > 1:1835944/1‑71 (MQ=255) aaTCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGAATc < 1:133914/71‑1 (MQ=255) aaTCGCTTTATGCTGCTATTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCTAAGCAACGGAATc > 1:1799453/1‑71 (MQ=255) aaTCGCTTTATGCTGCTATTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGAAt > 1:1797354/1‑70 (MQ=255) aaTCGCTTTATGCTGCTATTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGAATc > 1:1342141/1‑71 (MQ=255) aTCGCTTTATGCTGCTGTTGTCTACACTATATTCTCtt < 1:640390/38‑1 (MQ=255) gCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGAATCg > 1:1329760/1‑68 (MQ=255) aTGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGAATCGTTGCACg < 1:694807/70‑1 (MQ=255) aTGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGAATCGTTGCACg < 1:1717045/70‑1 (MQ=255) ctgctgTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGAATCGTTGCACGGTCg < 1:710834/71‑1 (MQ=255) ctgctgTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGAATCGTTGCACGGTCg < 1:1312875/71‑1 (MQ=255) ctgctgTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGAATCGTTGCACGGTCg < 1:1082623/71‑1 (MQ=255) gttgtCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGAATCGTTGCACGGTCGTacac > 1:510312/1‑71 (MQ=255) | GCGTGAACTTCTGCTTTCGGATGAATACGCAGAGCAAAAGCGAGCGGTCAATCGCTTTATGCTGCTGTTGTCTACACTATATTCTCTTGACGCCCAGGCGTTTGCCGAAGCAACGGAATCGTTGCACGGTCGTACAC > W3110S.gb/713624‑713760 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |