Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 907,713 G→A 100% T331I (ACC→ATC)  ybjT ← conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb907,7130GA87.5% 14.3 / ‑3.0 8T331I (ACC→ATC) ybjTconserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (3/4);  total (3/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGC  >  W3110S.gb/907656‑907774
                                                         |                                                             
ccgccgATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTg                                                  <  1:857696/71‑1 (MQ=255)
        ttGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCa                                           >  1:1035237/1‑70 (MQ=255)
                cTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCg                                  <  1:1424324/71‑1 (MQ=255)
                 tACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCg                                  <  1:361150/70‑1 (MQ=255)
                            aaGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGc                      >  1:1481261/1‑71 (MQ=255)
                                     ggCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCa             <  1:1568389/71‑1 (MQ=255)
                                     ggCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCa             <  1:807468/71‑1 (MQ=255)
                                                gTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGc  >  1:1118323/1‑71 (MQ=255)
                                                         |                                                             
CCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGC  >  W3110S.gb/907656‑907774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑