Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,303,631	C→T	100%	Q246Y (CAG→TAT)	oppA →	oligopeptide transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,303,631	0	C	T	100.0%	27.7 / NA	11	Q246Y (CAG→TAT)	oppA	oligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGT  >  W3110S.gb/1303566‑1303684                                                                  |                                                      tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                  <  1:306346/71‑1 (MQ=255)                     gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                 <  1:1058827/68‑1 (MQ=255)                               cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                                   >  1:1211401/1‑56 (MQ=255)                               cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                                   >  1:940819/1‑56 (MQ=255)                                     aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGACTa                                     >  1:1676663/1‑48 (MQ=38)                                      cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc              <  1:50865/70‑1 (MQ=255)                                      cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc              <  1:560394/70‑1 (MQ=255)                                         gAACAAGGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAcc          >  1:234122/1‑71 (MQ=255)                                         gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt                                    >  1:1074374/1‑45 (MQ=38)                                              acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGt                        <  1:1611893/52‑1 (MQ=255)                                                 cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt  <  1:799613/71‑1 (MQ=255)                                                                  |                                                      TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGT  >  W3110S.gb/1303566‑1303684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑