Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,303,633 G→T 100% Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,303,6330GT100.0% 28.6 / NA 11Q246Y (CAG→TAT) oppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (5/6);  total (5/6)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGT  >  W3110S.gb/1303566‑1303684
                                                                   |                                                   
tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                  <  1:306346/71‑1 (MQ=255)
                    gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                 <  1:1058827/68‑1 (MQ=255)
                              cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                                   >  1:1211401/1‑56 (MQ=255)
                              cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                                   >  1:940819/1‑56 (MQ=255)
                                    aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGACTa                                     >  1:1676663/1‑48 (MQ=38)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc              <  1:50865/70‑1 (MQ=255)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc              <  1:560394/70‑1 (MQ=255)
                                        gAACAAGGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAcc          >  1:234122/1‑71 (MQ=255)
                                        gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt                                    >  1:1074374/1‑45 (MQ=38)
                                             acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGt                        <  1:1611893/52‑1 (MQ=255)
                                                cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt  <  1:799613/71‑1 (MQ=255)
                                                                   |                                                   
TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGT  >  W3110S.gb/1303566‑1303684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑