| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,058,034 | C→T | 100% | G279G (GGC→GGT) | amn → | AMP nucleosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,058,034 | 0 | C | T | 80.0% | 38.7 / 6.0 | 20 | G279G (GGC→GGT) | amn | AMP nucleosidase |
| Reads supporting (aligned to +/- strand): ref base C (1/3); new base T (10/6); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTT > W3110S.gb/2057984‑2058099 | tGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTa > 1:1574302/1‑70 (MQ=255)tGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGATa > 1:1159597/1‑70 (MQ=255) ttAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCaaa < 1:358937/59‑1 (MQ=255) tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATg > 1:664337/1‑60 (MQ=255) tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAAcc < 1:751867/68‑1 (MQ=255) aTTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGa > 1:181527/1‑48 (MQ=255) ggTCAGGGTATTACTCTGGTGAATATTGGTGTGGGATCGTCAAATGCTAAAACCATCTGCGatcatc > 1:1821539/1‑67 (MQ=255) ggTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAAcc < 1:332519/54‑1 (MQ=255) gTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGc < 1:1458746/70‑1 (MQ=255) gTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGc < 1:619754/70‑1 (MQ=255) gTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGc < 1:744584/70‑1 (MQ=255) cAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGt > 1:502294/1‑71 (MQ=255) aCTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGccc > 1:1626494/1‑71 (MQ=255) aCTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGccc > 1:1801527/1‑71 (MQ=255) aaTATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTg > 1:1325248/1‑71 (MQ=255) aaTATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTg > 1:1257564/1‑71 (MQ=255) ttGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGtt > 1:1794204/1‑70 (MQ=255) ttGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTgg < 1:1805154/45‑1 (MQ=255) ttGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTgg < 1:425974/45‑1 (MQ=255) ttGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTgg < 1:1006161/45‑1 (MQ=255) | TGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTT > W3110S.gb/2057984‑2058099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |