| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,855,731 | G→T | 20.9% | intergenic (‑269/‑18) | ygbA ← / → mutS | hypothetical protein/methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,855,731 | 0 | G | T | 20.9% | 115.2 / 14.5 | 43 | intergenic (‑269/‑18) | ygbA/mutS | hypothetical protein/methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base G (21/13); new base T (6/3); total (27/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 | |||||||||||
CTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAG > W3110S.gb/2855662‑2855793 | cTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAggg < 1:82716/71‑1 (MQ=255) tCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAggg > 1:236011/1‑70 (MQ=255) tCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAggg > 1:1726378/1‑70 (MQ=255) tCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAggg < 1:1649766/70‑1 (MQ=255) aTGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACAt < 1:191349/71‑1 (MQ=255) cTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCAt < 1:1542766/71‑1 (MQ=255) cTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCAt < 1:694493/71‑1 (MQ=255) aTGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATg > 1:393028/1‑69 (MQ=255) aTGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAg > 1:288238/1‑71 (MQ=255) aTGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAg > 1:996929/1‑71 (MQ=255) tgATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGt > 1:1294966/1‑69 (MQ=255) gATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATaa > 1:1019432/1‑58 (MQ=255) gATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACAGGACATaa > 1:1755685/1‑58 (MQ=255) aTTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGa < 1:425408/46‑1 (MQ=255) aTTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGa < 1:1828281/46‑1 (MQ=255) tACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCaa > 1:1654048/1‑69 (MQ=255) tACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCaa > 1:1070050/1‑69 (MQ=255) tACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCaa > 1:445778/1‑69 (MQ=255) tACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCaa > 1:538337/1‑69 (MQ=255) aCAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCaa > 1:1345195/1‑68 (MQ=255) aaTAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTc < 1:849029/71‑1 (MQ=255) aaTAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTc < 1:1646539/71‑1 (MQ=255) aaTAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTc < 1:1794593/71‑1 (MQ=255) aaTAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTc < 1:1439213/71‑1 (MQ=255) aaTAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTc < 1:645186/71‑1 (MQ=255) aTAAAAACCATCACACCCCATTTAATATCAGGGAAc < 1:1703276/36‑1 (MQ=255) aTAAAAACCATCACACCCCATTTAATATCAGGGAAc < 1:1538741/36‑1 (MQ=255) cATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGacgccca < 1:305755/70‑1 (MQ=255) aatTTAATATCAGTAAACCGGACATAACCCatgaagaaaaatcgcgcttttttgaagtgggcagggggc > 1:1344135‑M1/2‑30 (MQ=255) aatTTAATATCAGTAAACCGGACATAACCCatgaagaaaaatcgcgcttttttgaagtgggcagggggc > 1:153931‑M1/2‑30 (MQ=255) aatTTAATATCAGTAAACCGGACATAACCCatgaagaaaaatcgcgcttttttgaagtgggcaggggg > 1:1044624‑M1/2‑30 (MQ=255) aTTTAATATCAGTAAACCGGACATAACCCatgaagaaaaatcgcgcttttttgaagtgggcag > 1:1361272‑M1/1‑29 (MQ=255) aTTTAATATCAGTAAACAGGACATAACCCatgaagaaaaatcgcgcttttttgaagtgggcagggggcaag > 1:1100104‑M1/1‑29 (MQ=255) ttAATATCAGTAAACCGGACATAACCCatgaagaaaaatcgcgcttttttgaagtgggcagggggcaagta > 1:1313994‑M1/1‑27 (MQ=255) ttAATATCAGGGAACCGGACATAAGCCCATGAGTGCAATAGAAAATTTCGacgccca > 1:891181/1‑57 (MQ=255) ttAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGacgccca > 1:230176/1‑57 (MQ=255) ttAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGacgccca > 1:549905/1‑57 (MQ=255) tAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGa > 1:681159/1‑50 (MQ=255) tAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCatgatg > 1:780951/1‑69 (MQ=255) aaTATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGacgcccata > 1:1815169/1‑57 (MQ=255) aaTATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGacgcccata > 1:685067/1‑57 (MQ=255) aaTATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGacgcccata > 1:1154887/1‑57 (MQ=255) aaTATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATgcag > 1:620312/1‑71 (MQ=255) aaTATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATgcag > 1:119299/1‑71 (MQ=255) tatCAGTAAACCGGACATAACCCatgaagaaaaatcgcgcttttttga < 1:319097‑M1/48‑26 (MQ=255) tatCAGTAAACCGGACATAACCCatgaagaaaaatcgcgcttttttga < 1:489123‑M1/48‑26 (MQ=255) tatCAGTAAACCGGACATAACCCatgaagaaaaatcgcgcttttttga < 1:1321590‑M1/48‑26 (MQ=255) | CTCCGGTATCATGTGCGCCTTATGTGATTACAACGAAAATAAAAACCATCACACCCCATTTAATATCAGGGAACCGGACATAACCCCATGAGTGCAATAGAAAATTTCGACGCCCATACGCCCATGATGCAG > W3110S.gb/2855662‑2855793 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |