| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,141,782 | G→T | 16.6% | P347T (CCG→ACG) | hybB ← | predicted hydrogenase 2 cytochrome b type component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,141,782 | 0 | G | T | 16.6% | 85.6 / 4.7 | 30 | P347T (CCG→ACG) | hybB | predicted hydrogenase 2 cytochrome b type component |
| Reads supporting (aligned to +/- strand): ref base G (16/9); new base T (2/3); total (18/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 | |||||||||||
TGAGTACGATGTAAGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGAATAGGTCAGACGCCAGGTTG > W3110S.gb/3141713‑3141844 | tGAGTACGATGTAAGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCggg < 1:926930/71‑1 (MQ=255)tGAGTACGATGTAAGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCggg < 1:807802/71‑1 (MQ=255) cGATGTAAGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTa < 1:1721159/70‑1 (MQ=255) tGTAAGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAg < 1:639658/65‑1 (MQ=255) tGTAAGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAg < 1:1303888/65‑1 (MQ=255) tGTAAGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAg < 1:1452738/65‑1 (MQ=255) aGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTa > 1:1614497/1‑63 (MQ=255) aGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAAc < 1:1808988/71‑1 (MQ=255) aGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAAc < 1:158982/71‑1 (MQ=255) tctcAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCCGccc > 1:388239/1‑71 (MQ=255) tctcAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCCGccc > 1:897050/1‑71 (MQ=255) tAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCCGCCCGGGTTg < 1:558462/71‑1 (MQ=255) tAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCCGCCCGGGTTg < 1:539792/71‑1 (MQ=255) gCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAAc > 1:1788800/1‑55 (MQ=255) gCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCCGCCCGGGTTGaa > 1:1434737/1‑71 (MQ=255) caAAACCAATAGAAATCAACAGTTCTTCCCAGGTCggg < 1:934157/38‑1 (MQ=255) aaaCCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTa > 1:751769/1‑41 (MQ=255) aaaCCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTa > 1:25456/1‑41 (MQ=255) aaaCCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTa > 1:1509649/1‑41 (MQ=255) aaaCCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTa > 1:129771/1‑41 (MQ=255) tAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGaa > 1:43713/1‑71 (MQ=255) tAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGaa > 1:1048288/1‑71 (MQ=255) tAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGa > 1:369171/1‑70 (MQ=255) tAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGa > 1:873424/1‑70 (MQ=255) aaCAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGAATAGGt > 1:237494/1‑68 (MQ=255) aaCAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGAATAGGt > 1:235312/1‑68 (MQ=255) aaCAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGAATAGGt > 1:811349/1‑68 (MQ=255) gTTCTTCCCAGGTCGTGAAGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGAATAGGTCAGACGc > 1:275309/1‑71 (MQ=255) gTTCTTCCCAGGTCGTGAAGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGAATAGGTCAGACGc > 1:1223536/1‑71 (MQ=255) ttcCCAGGTCGGGATGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGAATAGGTCAGACGCCa < 1:257456/69‑1 (MQ=255) ccAGGTCGTGAAGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGAATAGGTCAGACGCCAGGTTg < 1:1586247/71‑1 (MQ=255) ccAGGTCGTGAAGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGAATAGGTCAGACGCCAGGTTg < 1:827287/71‑1 (MQ=255) ccAGGTCGTGAAGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGAATAGGTCAGACGCCAGGTTg < 1:1251219/71‑1 (MQ=255) | TGAGTACGATGTAAGCGCAAATCTCAATAGCCACAAAACCAATAGAAATCAACAGTTCTTCCCAGGTCGGGAAGTAGGCGTAACCGCCGCCCGGGTTGAATGCCACCAGCGAATAGGTCAGACGCCAGGTTG > W3110S.gb/3141713‑3141844 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |