Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,315,139 T→A 100% D244V (GAT→GTT)  infB ← fused protein chain initiation factor 2, IF2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,315,1390TA94.7% 48.3 / ‑5.0 18D244V (GAT→GTT) infBfused protein chain initiation factor 2, IF2
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (12/5);  total (13/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGT  >  W3110S.gb/3315070‑3315179
                                                                     |                                        
ttgttgCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATc                                         >  1:1613537/1‑71 (MQ=255)
        tttCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCg                                  >  1:1490275/1‑70 (MQ=255)
        tttCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCg                                  >  1:1598808/1‑70 (MQ=255)
        tttCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGt                                 >  1:1492366/1‑71 (MQ=255)
        tttCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGt                                 >  1:1185838/1‑71 (MQ=255)
             tcGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGtct                               >  1:355284/1‑68 (MQ=255)
                     gctgctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGc                     >  1:1662865/1‑70 (MQ=255)
                         ctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCa                <  1:908856/71‑1 (MQ=255)
                         ctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCa                <  1:1388454/71‑1 (MQ=255)
                         ctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCa                <  1:1511202/71‑1 (MQ=255)
                           ttCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGc                 >  1:784533/1‑68 (MQ=255)
                                   aCGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAag      >  1:1825326/1‑71 (MQ=255)
                                    cGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAag      >  1:1738035/1‑70 (MQ=255)
                                       ccacggccacggCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAagtag   >  1:337859/1‑70 (MQ=255)
                                                   ccgccTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTgaga        <  1:1348305/53‑1 (MQ=255)
                                                     gccTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAag      >  1:459398/1‑53 (MQ=255)
                                                     gccTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAag      >  1:332173/1‑53 (MQ=255)
                                                              ttCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAagtagt  <  1:705843/48‑1 (MQ=255)
                                                                     |                                        
TTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGT  >  W3110S.gb/3315070‑3315179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑