| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,688,001 | A→G | 66.7% | S440G (AGT→GGT) | yifB → | predicted bifunctional protein, enzyme and transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,688,001 | 0 | A | G | 66.7% | 24.5 / 18.4 | 24 | S440G (AGT→GGT) | yifB | predicted bifunctional protein, enzyme and transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (4/4); new base G (14/2); total (18/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAACAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGA > W3110S.gb/3687932‑3688067 | aaaCAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAg > 1:1424368/1‑71 (MQ=255)aaaCAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAg > 1:1612439/1‑71 (MQ=255)aaaCAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAg > 1:211907/1‑71 (MQ=255) gccgccAGAGAGCGCCAATTTAAGAGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGcc > 1:1402103/1‑70 (MQ=255) agagagCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCt > 1:596344/1‑70 (MQ=255) cAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGaaa < 1:802048/49‑1 (MQ=255) aTTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATa > 1:1319466/1‑49 (MQ=255) tAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGc > 1:1815780/1‑70 (MQ=255) tAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGc > 1:467368/1‑70 (MQ=255) ggCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGa < 1:1830340/67‑1 (MQ=255) cAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGa < 1:1160658/65‑1 (MQ=255) tAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGAtgt > 1:859580/1‑71 (MQ=255) tAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGAtgt > 1:451577/1‑71 (MQ=255) aaCTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGAtgtg < 1:1788864/70‑1 (MQ=255) tGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGc > 1:972233/1‑61 (MQ=255) tGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGc > 1:927267/1‑61 (MQ=255) tGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGc > 1:584207/1‑61 (MQ=255) aaTGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGaa > 1:1748204/1‑54 (MQ=255) aaTGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGa > 1:679766/1‑56 (MQ=255) aaTGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGa > 1:1326338/1‑56 (MQ=255) aTGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGa > 1:1545771/1‑55 (MQ=255) ccTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGa < 1:1402727/49‑1 (MQ=255) gctggATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAacac > 1:1233388/1‑71 (MQ=255) gATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGa < 1:695967/70‑1 (MQ=255) | AAACAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGA > W3110S.gb/3687932‑3688067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |