Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,374 C→T 100% pseudogene (507/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,3740CT100.0% 94.4 / NA 32pseudogene (507/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/16);  total (16/16)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTG  >  W3110S.gb/3824315‑3824437
                                                           |                                                               
cTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg                                                      >  1:1128407/1‑71 (MQ=255)
cTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg                                                      >  1:267940/1‑71 (MQ=255)
cTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTg                                                      >  1:1829888/1‑71 (MQ=255)
        cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                              <  1:692401/71‑1 (MQ=255)
        cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                              <  1:690142/71‑1 (MQ=255)
                 gAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGt                                       >  1:764295/1‑69 (MQ=255)
                  aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                     >  1:681826/1‑70 (MQ=255)
                  aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                     >  1:248900/1‑70 (MQ=255)
                  aaaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTg                                     >  1:1830059/1‑70 (MQ=255)
                    aGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAg                                  <  1:1134052/71‑1 (MQ=255)
                     gggATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                           <  1:200206/45‑2 (MQ=255)
                     gggATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                           <  1:99291/45‑2 (MQ=255)
                     gggATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                           <  1:708658/45‑2 (MQ=255)
                     gggATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                           <  1:34742/45‑2 (MQ=255)
                     gggATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                           <  1:1491618/45‑2 (MQ=255)
                      ggATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGAc                                <  1:61938/71‑1 (MQ=255)
                           gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc                           <  1:1386288/71‑1 (MQ=255)
                                 gCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGActca                             >  1:1395535/1‑62 (MQ=255)
                                      ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGttaa                                    >  1:1387703/1‑49 (MQ=255)
                                      ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                   >  1:112359/1‑52 (MQ=255)
                                      ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                   >  1:1802188/1‑52 (MQ=255)
                                      ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                   >  1:1485878/1‑52 (MQ=255)
                                      ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                   >  1:46528/1‑52 (MQ=255)
                                      ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                   >  1:1114141/1‑52 (MQ=255)
                                        cTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa               <  1:373701/70‑1 (MQ=255)
                                                aTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                   >  1:777412/1‑42 (MQ=25)
                                                aTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                                   >  1:81366/1‑42 (MQ=25)
                                                    taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGtg  <  1:1499980/69‑1 (MQ=255)
                                                    taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt   <  1:523122/68‑1 (MQ=255)
                                                    taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt   <  1:1493117/68‑1 (MQ=255)
                                                    taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt   <  1:1382644/68‑1 (MQ=255)
                                                    taaaCGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACGTAGt   <  1:1572941/68‑1 (MQ=255)
                                                           |                                                               
CTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACGTAGTG  >  W3110S.gb/3824315‑3824437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: