| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,195,173 | A→G | 100% | *118* (TAA→TGA) | rplR → | 50S ribosomal subunit protein L18 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,195,173 | 0 | A | G | 100.0% | 64.7 / NA | 19 | *118* (TAA→TGA) | rplR | 50S ribosomal subunit protein L18 |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (7/12); total (7/12) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAATATCATGGTCGTGTCCAGGCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTAAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCTGGCGAACTGCAGGAAAAGCTGATCGCG > W3110S.gb/4195112‑4195239 | cAATATCATGGTCGTGTCCAGGCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTagag < 1:552959/70‑1 (MQ=255) gTCGTGTCCAGGCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGATg < 1:1609559/70‑1 (MQ=255) ggCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGATGGCTca > 1:755600/1‑65 (MQ=255) ggCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGATGGCTca > 1:723361/1‑65 (MQ=255) ggCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGATGGCTca > 1:493803/1‑65 (MQ=255) ggCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGATGGCTca > 1:301421/1‑65 (MQ=255) ggCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGATGGCTca > 1:102891/1‑65 (MQ=255) gATGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCt < 1:1683409/71‑1 (MQ=255) gATGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCt < 1:869995/71‑1 (MQ=255) gATGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCt < 1:190271/71‑1 (MQ=255) aTGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGa < 1:1698871/47‑1 (MQ=255) aTGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGa < 1:1699680/47‑1 (MQ=255) aTGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGa < 1:1511926/47‑1 (MQ=255) aTGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGa < 1:1190463/47‑1 (MQ=255) cTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACa < 1:1103744/51‑1 (MQ=255) cTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCTGGCGAACTGCAGGaaa < 1:1619549/71‑1 (MQ=255) cAGTTCTGAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCTGGCGaa > 1:166683/1‑53 (MQ=255) aGTTCTGAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCTGGCGAACTGCAGGAAAAGCTGATcg > 1:1111701/1‑71 (MQ=255) ttCTGAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCTGGCGAACTGCAGGAAAAGCTGATcgcg < 1:1291787/71‑1 (MQ=255) | CAATATCATGGTCGTGTCCAGGCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTAAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCTGGCGAACTGCAGGAAAAGCTGATCGCG > W3110S.gb/4195112‑4195239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |