Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,246,990 C→T 100% R40H (CGT→CAT)  malG ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,246,9900CT80.0% 16.1 / 0.3 10R40H (CGT→CAT) malGmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (5/3);  total (6/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAG  >  W3110S.gb/4246938‑4247045
                                                    |                                                       
aTCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgacga                                       >  1:1821729/1‑71 (MQ=255)
                          ggCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCt             <  1:1839397/71‑1 (MQ=255)
                             tGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACAt                    >  1:1290987/1‑61 (MQ=255)
                             tGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACAt                    >  1:19565/1‑61 (MQ=255)
                             tGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACAt                    >  1:220100/1‑61 (MQ=255)
                                  gTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa     <  1:853465/71‑1 (MQ=255)
                                  gTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa     <  1:866988/71‑1 (MQ=255)
                                   tCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaaa    <  1:646857/71‑1 (MQ=255)
                                       aaaGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAag  >  1:1679737/1‑69 (MQ=255)
                                       aaaGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAag  >  1:856825/1‑69 (MQ=255)
                                                    |                                                       
ATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAG  >  W3110S.gb/4246938‑4247045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑