| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,340,671 | G→A | 100% | T347T (ACC→ACT) | adiC ← | arginine:agmatin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,340,671 | 0 | G | A | 77.8% | 32.2 / 5.6 | 18 | T347T (ACC→ACT) | adiC | arginine:agmatin |
| Reads supporting (aligned to +/- strand): ref base G (3/1); new base A (8/6); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCACAGGTGTAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACCGACGATAA > W3110S.gb/4340603‑4340729 | gcACAGGTGTAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGt > 1:545844/1‑71 (MQ=255) caGGTGTAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTcgc > 1:1472603/1‑71 (MQ=255) gtgtAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTcg > 1:117919/1‑67 (MQ=255) gtgtAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGtt > 1:1351071/1‑71 (MQ=255) aaGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTg < 1:119769/71‑1 (MQ=255) atatGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTcgc < 1:685596/60‑1 (MQ=255) aaGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAAtgc > 1:1591996/1‑58 (MQ=255) aaGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAAtg > 1:1528163/1‑57 (MQ=255) aaGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTgg > 1:767505/1‑69 (MQ=255) aaGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTgg > 1:1836116/1‑69 (MQ=255) gACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATgg > 1:579940/1‑71 (MQ=255) gACCGAGACGGAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATgg > 1:547312/1‑71 (MQ=255) cGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCaa < 1:389293/70‑1 (MQ=255) ggAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCaaaa < 1:1618089/71‑1 (MQ=255) ggAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCaaaa < 1:5472/71‑1 (MQ=255) ggAAGAAACCAGACCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCaaaa < 1:1088944/71‑1 (MQ=255) aCCGAACTCTTTAGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACCGACGATaa < 1:1003676/71‑1 (MQ=255) ctctTTAGTCGCGTTTGGTGAAATGCTCCTGAGCTGGAAGATg > 1:1518779/1‑43 (MQ=255) | GCACAGGTGTAAAGATATGGCACCAGTGTAAAGATGACCGAGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCTGGAAGATGGTCATCAAAATACCGACGATAA > W3110S.gb/4340603‑4340729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |