Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,416,062 A→G 100% N27D (AAT→GAT)  yjfK → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,416,0620AG81.8% 21.1 / ‑0.1 11N27D (AAT→GAT) yjfKconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (6/3);  total (8/3)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGC  >  W3110S.gb/4416020‑4416123
                                          |                                                             
aataaGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGTCACGTTAGc                                   >  1:1771728/1‑71 (MQ=255)
            aTCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCt                       >  1:1057165/1‑71 (MQ=255)
            aTCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCt                       >  1:1669514/1‑71 (MQ=255)
               gCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTgg                     >  1:1013023/1‑70 (MQ=255)
                      gTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGa              <  1:1062259/70‑1 (MQ=255)
                      gTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGa              <  1:1233960/70‑1 (MQ=255)
                      gTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGa              <  1:1338169/70‑1 (MQ=255)
                         cGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGc                                   >  1:1139055/1‑46 (MQ=255)
                         cGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGc                                   >  1:1588608/1‑46 (MQ=255)
                         cGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGc                                   >  1:1642029/1‑46 (MQ=255)
                                 cTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTgc  >  1:1728962/1‑71 (MQ=255)
                                          |                                                             
AATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGC  >  W3110S.gb/4416020‑4416123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑