| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,434,349 | T→A | 100% | intergenic (+112/‑197) | fklB → / → cycA | FKBP‑type peptidyl‑prolyl cis‑trans isomerase/D‑alanine/D‑serine/glycine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,434,349 | 0 | T | A | 81.1% | 84.3 / 15.2 | 37 | intergenic (+112/‑197) | fklB/cycA | FKBP‑type peptidyl‑prolyl cis‑trans isomerase/D‑alanine/D‑serine/glycine transporter |
| Reads supporting (aligned to +/- strand): ref base T (7/0); new base A (10/20); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.89e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAA > W3110S.gb/4434288‑4434407 | aaaCCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCt > 1:899866/1‑71 (MQ=255) aaCCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTg > 1:807829/1‑71 (MQ=255) aaCCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTg > 1:219373/1‑71 (MQ=255) aaCCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTg > 1:217121/1‑71 (MQ=255) cccTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATtg > 1:1830331/1‑62 (MQ=255) ttGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTAt > 1:1341775/1‑71 (MQ=255) tGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTGTTTcg > 1:1629753/1‑64 (MQ=255) cGGCGCTATTTTGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGa < 1:1142345/71‑1 (MQ=255) cGGCGCTATTTTGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGa < 1:867573/71‑1 (MQ=255) gcTATTTTGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGa < 1:1304568/67‑1 (MQ=255) gcTATTTTGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGa < 1:1387459/67‑1 (MQ=255) gcTATTTTGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGa < 1:1676389/67‑1 (MQ=255) ttttGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGc < 1:10088/42‑1 (MQ=255) ttttGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGc < 1:124547/42‑1 (MQ=255) ttttGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGc < 1:1375813/42‑1 (MQ=255) ttGTGTTGCAAAGACCCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:1198019/1‑71 (MQ=255) ttGTGTTGCAAAGACCCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:280163/1‑71 (MQ=255) ttGTGTTGCAAAGACCCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:450784/1‑71 (MQ=255) ttGTGTTGCAAAGACCCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:967817/1‑71 (MQ=255) ttGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:1224264/1‑70 (MQ=255) ttGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGacac > 1:617809/1‑69 (MQ=255) ttGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:7023/1‑70 (MQ=255) gtgtTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcactcac > 1:1197399/1‑71 (MQ=255) tgtTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcactcac < 1:317967/70‑1 (MQ=255) tgtTGCAAAGA‑CCCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcactcac < 1:315758/70‑1 (MQ=255) cGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAAtt < 1:454850/71‑1 (MQ=255) aGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGata > 1:1087769/1‑42 (MQ=255) aGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGata > 1:1813661/1‑42 (MQ=255) cGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTaa < 1:1708536/67‑1 (MQ=255) cGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTaa < 1:1519238/67‑1 (MQ=255) cGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTaa < 1:1379394/67‑1 (MQ=255) cGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTaa < 1:822424/67‑1 (MQ=255) gtATTATTGTGAGCTGTTTCGCGTTATCACCGTGatat < 1:1768252/38‑1 (MQ=255) gtATTATTGTGAGCTGTTTCGCGTTATCACCGTGatat < 1:1754005/38‑1 (MQ=255) gtATTATTGTGAGCTGTTTCGCGTTATCACCGTGatat < 1:1610192/38‑1 (MQ=255) gtATTATTGTGAGCTGTTTCGCGTTATCACCGTGatat < 1:896978/38‑1 (MQ=255) gtATTATTGTGAGCTGTTTCGCGTTATCACCGTGatat < 1:1196385/38‑1 (MQ=255) tattatTGTGAGCTGTTTCGCGTTATCAGCGTGATATGACACTCACTTTAAACATaa > 1:1255835/1‑57 (MQ=39) tattatTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATaa > 1:666945/1‑57 (MQ=255) tattatTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATaa > 1:1392242/1‑57 (MQ=255) tattatTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATaa > 1:719704/1‑57 (MQ=255) tattatTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATaa > 1:725824/1‑57 (MQ=255) tattatTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATa > 1:1284462/1‑56 (MQ=255) | AAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑CCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAA > W3110S.gb/4434288‑4434407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |