| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,563,589 | T→A | 100% | K207M (AAG→ATG) | iadA ← | isoaspartyl dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0% | 60.4 / NA | 21 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (13/8); total (13/8) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563532‑4563654 | agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc < 1:419980/71‑1 (MQ=255)agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc < 1:373323/71‑1 (MQ=255)agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc < 1:1505049/71‑1 (MQ=255) cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:629658/71‑1 (MQ=255) cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:1803806/71‑1 (MQ=255) cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:464747/1‑53 (MQ=255) acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:750029/71‑1 (MQ=255) acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:349904/71‑1 (MQ=255) cGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:1083297/1‑45 (MQ=255) aGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCa < 1:1497599/71‑1 (MQ=255) aaTAGGCTTTAACGCCATTTTACTGTCGCCCATGTGGAac > 1:799343/1‑40 (MQ=38) aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa > 1:1222585/1‑39 (MQ=255) aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAac > 1:1247709/1‑40 (MQ=255) tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:461319/1‑38 (MQ=255) tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt > 1:106101/1‑38 (MQ=255) tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc > 1:1624494/1‑50 (MQ=255) tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc > 1:569390/1‑50 (MQ=255) tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc > 1:1088484/1‑50 (MQ=255) aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg > 1:1207964/1‑43 (MQ=255) aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg > 1:1108220/1‑43 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg > 1:1660680/1‑71 (MQ=255) | AGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563532‑4563654 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |