| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,109 | T→C | 60.8% | T241T (ACT→ACC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,109 | 0 | T | C | 60.8% | 40.4 / 56.5 | 51 | T241T (ACT→ACC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (4/16); new base C (22/9); total (26/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.31e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGC > W3110S.gb/71040‑71173 | gTTAGGGATTAGCGTCTTAAGCTGGCGCGATGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTa < 1:1495630/71‑1 (MQ=255)gTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTa < 1:1615796/71‑1 (MQ=255)gTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTa < 1:155394/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:1471141/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:1382576/71‑1 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGcc < 1:612425/70‑1 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTa > 1:816799/1‑70 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTAt > 1:1820069/1‑71 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTAt > 1:1606542/1‑71 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTAt > 1:816946/1‑71 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTAt > 1:1095677/1‑71 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:1251758/61‑1 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:401953/61‑1 (MQ=255) cTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:171921/60‑1 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGa > 1:1280168/1‑61 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGa > 1:519084/1‑61 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGc > 1:22384/1‑71 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGc > 1:1580394/1‑71 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGc > 1:952835/1‑71 (MQ=255) ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGa > 1:931581/1‑61 (MQ=255) ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGc > 1:689862/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGcc < 1:434297/70‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGc < 1:1478267/69‑1 (MQ=255) aGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggtcg > 1:1797031/1‑70 (MQ=255) aGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggtcg > 1:1244049/1‑70 (MQ=255) aGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGc < 1:1111290/71‑1 (MQ=255) gCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGt < 1:1155635/71‑1 (MQ=255) ttAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCt < 1:787514/41‑1 (MQ=255) ttAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCt < 1:580991/41‑1 (MQ=255) ttAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCt < 1:49073/41‑1 (MQ=255) agccAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:154360/4‑71 (MQ=255) agccAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:278010/4‑71 (MQ=255) aGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTg < 1:65356/70‑1 (MQ=255) ccAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACg < 1:967638/70‑1 (MQ=255) ccAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACg < 1:290148/70‑1 (MQ=255) gCGAAGCTGCTTTTGAGCACCACCCGGATGCCTAt > 1:954661/1‑35 (MQ=255) gctgctTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAActc > 1:1223096/1‑70 (MQ=255) ttttGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAt < 1:345021/68‑1 (MQ=255) ttttGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAt < 1:1128498/68‑1 (MQ=255) ttttGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAt < 1:1289768/68‑1 (MQ=255) ttttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGGTCAACTCTAtt < 1:1255243/69‑1 (MQ=255) ttttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAtt < 1:1000551/69‑1 (MQ=255) ttttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAtt < 1:1067465/69‑1 (MQ=255) ttGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTtctc > 1:222444/1‑71 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCgtcggtc > 1:769225/1‑37 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGttt > 1:526502/1‑50 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTtc > 1:1369061/1‑69 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTtc > 1:813131/1‑69 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTtc > 1:419078/1‑69 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTtc > 1:1770263/1‑69 (MQ=255) gCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcgc > 1:1673627/1‑69 (MQ=255) caccacCCGGATGCCTATCGCCACCGTCGGTCTCAATGTTGGTTTTGACGATCAACTCTAt < 1:446539/61‑1 (MQ=255) caccacCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAt < 1:57943/61‑1 (MQ=255) | GTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGC > W3110S.gb/71040‑71173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |