| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,175 | A→G | 42.9% | R263R (CGA→CGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,175 | 0 | A | G | 42.9% | 19.3 / 62.4 | 49 | R263R (CGA→CGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (12/16); new base G (10/11); total (22/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATG > W3110S.gb/71110‑71242 | aCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTAtt > 1:1750663/1‑71 (MQ=255)aCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGAGAGTAtt > 1:518419/1‑71 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:1787339/71‑1 (MQ=255) tGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa > 1:1083469/1‑71 (MQ=255) tGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaaaa < 1:1537762/71‑1 (MQ=255) ccTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATg > 1:526864/1‑71 (MQ=255) ccTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATg > 1:150117/1‑71 (MQ=255) ccTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATg > 1:1490740/1‑71 (MQ=255) tATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATg < 1:551496/69‑1 (MQ=255) tATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATg < 1:1839505/69‑1 (MQ=255) tATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATg < 1:918553/69‑1 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCAcc > 1:1612389/1‑71 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCAcc > 1:510005/1‑71 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCgg < 1:1110010/71‑1 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa < 1:143453/50‑1 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa < 1:551546/50‑1 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa < 1:165070/50‑1 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa < 1:543484/50‑1 (MQ=255) aTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcgag > 1:1542371/1‑71 (MQ=255) aTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcgag > 1:267294/1‑71 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaa < 1:947599/43‑1 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaa < 1:1774848/43‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttc < 1:735374/71‑2 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttc > 1:971182/1‑70 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaaaa > 1:1222112/1‑44 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaaaa > 1:1307617/1‑44 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:1299293/42‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:521714/42‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:764148/42‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:1319137/42‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:926595/42‑1 (MQ=255) ggTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCg > 1:1108031/1‑71 (MQ=255) gTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCg < 1:600954/70‑1 (MQ=255) tCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGc > 1:1307406/1‑46 (MQ=255) tCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg < 1:805096/71‑1 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:312466/1‑70 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:1279002/1‑70 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgt > 1:1092223/1‑69 (MQ=255) tctATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCcgagcga < 1:760220/49‑1 (MQ=255) tctATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCcgagcga < 1:154075/49‑1 (MQ=255) tctATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTtgtg < 1:1332187/66‑1 (MQ=255) tATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa > 1:1710151/1‑70 (MQ=255) tATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTtg > 1:355384/1‑62 (MQ=255) aTTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa > 1:1552660/1‑69 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:1376961/68‑1 (MQ=255) ctcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTg > 1:1253016/1‑70 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:953538/71‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAat < 1:1489509/70‑1 (MQ=255) gcgAGTATTTAAAAAATGCACCGGGGCCAGCCAGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:1096750/71‑1 (MQ=255) | ACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATG > W3110S.gb/71110‑71242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |