| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,911,008 | 0 | G | A | 63.6% | 96.5 / 212.8 | 305 | intergenic (+34/‑38) | xylA/xylB | D‑xylose isomerase/xylulokinase |
| Reads supporting (aligned to +/- strand): ref base G (0/111); new base A (194/0); total (194/111) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 0.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 305 total aligned reads displayed.
TTCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAT > W3110S.gb/3910963‑3911063 | ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCTGTTATCAGTAGCGATAc > 1:162100/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGa > 1:669756/1‑53 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGAt > 1:555093/1‑54 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATa > 1:322241/1‑55 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:454731/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:532685/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:524718/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:520021/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:507970/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:498065/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:479545/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:476761/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:473689/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:46633/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:391410/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:447032/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:440736/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:430056/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:416213/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:410550/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:401576/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:110549/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:634971/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:88702/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:83658/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:72189/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:708869/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:706798/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:694723/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:680563/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:666223/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:553373/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:623450/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:614357/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:600256/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:590414/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:580729/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:570210/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:564486/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:561510/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:198605/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:27630/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:272385/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:268093/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:2630/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:258718/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:384769/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:235775/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:228731/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:225804/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:220324/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:248878/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:191049/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:172446/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:148577/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:144528/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:129969/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:125972/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:12442/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:117374/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:101636/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:284872/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:353622/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:295639/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:306424/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:315292/1‑56 (MQ=255)ttCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCAGTAGCGATAc > 1:324968/1‑56 (MQ=255) gTTCTCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:641350/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:405669/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:654770/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:662151/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:427743/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:336996/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:347168/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:684790/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:688857/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:691544/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:133125/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:186056/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:404664/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:713519/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:395108/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:85015/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:377162/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:652067/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:64270/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:518543/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:33123/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:432533/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:606063/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:213057/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:329760/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:574204/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:318792/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:311105/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:540562/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:49089/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAt < 1:282414/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATAGGGATAGAt < 1:546157/62‑1 (MQ=255) gTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGACCGATATGTATATCGGGATAGAt < 1:530145/62‑1 (MQ=255) | TTCGACAAATAACGGCTAACTGTGCAGTCCGTTGGCCCGGTTATCGGTAGCGATACCGGGCATTTTTTTAAGGAACGATCGATATGTATATCGGGATAGAT > W3110S.gb/3910963‑3911063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |